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81 notecards = 21 pages (4 cards per page)

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Micro: Gram Negative Bacilli of Medical Importance

front 1

what is the only way that shigella and e. coli are distinguished

back 1

shigella cause bacillary dysentery, e. coli do not

front 2

how many serogroups of shigella are there

back 2

4

front 3

what are the serogroups of shigella based on

back 3

O carbohydrate antigens

front 4

are there animal cariers of shigella

back 4

no

front 5

where does shigella live

back 5

colon

front 6

how is shigella transmitted

back 6

fecal oral

front 7

what does shigella invade in the colon

back 7

mucosa, rarely past epitheilal cells

front 8

is shigella septicemia rate high

back 8

no

front 9

what does severity of shigella illness depend on

back 9

serogroup cuasing infection

front 10

what is the milder shigella species

back 10

snonei

front 11

what is the more severe shigella species

back 11

s. dysentriae

front 12

how many cases of shigella are due to s. sonnei

back 12

75%

front 13

how many causes do children under 5 account for

back 13

50%

front 14

what is the correlation between shigella disease severity and amount of toxin produced

back 14

more toxin, more severe disease

front 15

what types of effects does shiga toxin have

back 15

neurotoxic
cytotoxic
enterotoxc

front 16

what are the parts of the shiga toxin

back 16

A and 5 B subunits

front 17

how many parts of the A subunit are there in shiga toxin

back 17

A1 and A2

front 18

what does the A1 peptides of shiga toxin do

back 18

irreversibly inactivates 60S ribosomal subunit resulting in inhibition or protein synthesis

front 19

what is the role of B subunit in shiga toxin

back 19

binding of toxin to mucosal cell

front 20

what is the role of shiga toxin in disease

back 20

unclear as toxin-negative strains can still cause disease

front 21

what will the gram stains of shigella look like

back 21

GNR, non lactose fermentes

front 22

is there H2S production by shigella

back 22

no

front 23

what type of biochemical tests/serology can be used to ID shigella

back 23

anti-O antibody agglutination

front 24

what types of stool exam would ID shigella

back 24

methylene blue: presence of PMNs suggests invasive organism like salmonella, shigella, campylobacter

front 25

what are the treatments for shigella

back 25

antibiotics can help, public health measures

front 26

what family is just like the salmonella family in genetics

back 26

arizona

front 27

where did arizona and salmonella have a commmon ancestor

back 27

s. enterica

front 28

what are the 3 main types of salmonella

back 28

typhi
choleraesuis
enteritidis

front 29

which type of salmonella only infects humans

back 29

typhi

front 30

how many serotypes are there of salmonella tyhphi and choleraesuis

back 30

1 each

front 31

how many salmonella enteritidis serotypes are there

back 31

shit ton

front 32

what is the clincal manifestation of s. typhii

back 32

typhoid fever

front 33

what happens in typhoid fever

back 33

fever
delirium
abdomen tender
enlarged spleen
"rose spots"

front 34

what are the rose spots of typhoid fever

back 34

rose colored papules on abdomen

front 35

how long does tyhpoid fever last

back 35

3-4 weeks

front 36

how many of typhii infected become chronic carriers

back 36

3%

front 37

how do typhii infected people become chronic carriers

back 37

GB infection

front 38

how is typhii tranmitted

back 38

fecal-oral

front 39

where does infection of typhii begin

back 39

SI, but few GI symptoms

front 40

where does typhii multiply

back 40

Peyer's patches

front 41

how does typhii spread

back 41

reticuloendotheila system in liver ans pleen

front 42

what is fever in typhii due to

back 42

bacteremia and endotoxin

front 43

where can s. typhii survive and grow

back 43

inside MPs

front 44

what is a virulence factor of s. typhii

back 44

capsule

front 45

what is improtant to defend vs. s. typhii infection

back 45

gastric acid

front 46

what will gram stain show with typhii

back 46

GNR
non lactose fermenter

front 47

does s. typhii make H2S

back 47

yes small

front 48

how would you identify s. typhii species

back 48

serologic tests

front 49

how would you treat typhii

back 49

quinolones, chloramphenico

front 50

how would you prevent typhii

back 50

clean water supply

front 51

what are the s. typhii vaccines

back 51

killed whole bacterial vaccine

live attenuated oral vaccine

killed purified vaccine

front 52

what are the clinical manifestationso f s. enteritidis/cholerasuis

back 52

gastroenteritis
septicemia
enteric fevers

front 53

how long after ingesting s. enteritids will you get an illness

back 53

18-24 hours

front 54

what are the symptoms of s. enteritids gastroenteritis

back 54

diarrhea, fever, abdrominal pain

front 55

how long does s. enteriditis gastroenteritis last

back 55

2-5 days

front 56

will s. enteritis remain in the colon after an acute infection

back 56

yes for a couple weeks

front 57

what are symptoms of s. enteritidis speticemia

back 57

prolonged fever chills anorexia anemia

front 58

what are the secondary infections of s. enteritiis septicemia

back 58

osteomyelitis
pneumonia
pulmonary abscess
meningitis
endocarditis

front 59

is there gastroenteritis in septicemia s. enteritidis

back 59

minor or absent

front 60

what is s. cholerasuis associated with

back 60

septicemia and osteomyelitis in sickle cell patients

front 61

what are s. enteritidis enteric fever symptoms similar to

back 61

typhoid fever but milder and lower mortality

front 62

what are the symptoms of s. enteritidis enteric fever

back 62

initial lethargy, fever, malaise, myalgia

later: bacteremia with high fever and delirium

can develop rose spots and tender abdomen

front 63

what time will s. enteritidis enteric fever resolve in

back 63

3-4 weeks

front 64

what could be at the end of a s. enteridisis enteric fever

back 64

intestinal performation

front 65

where does s. enteritidis reside

back 65

enteric tracts of animals and humans

front 66

what are the most frequent animal sources of s. enteriditis

back 66

chickens, eggs, pet reptiles

front 67

what is the transmission route of s. enteritidis

back 67

fecal oral

front 68

what does s. enteritis invade

back 68

small and large intestine then blood to cause sepsis in 5-10%

front 69

what are the virulence factors of s. enteritis

back 69

endotoxin

front 70

what is an important host defense against s. enteritidis

back 70

gastric acid

front 71

what would a gram stain of s. enteritids look like

back 71

GNR
non lactose fermenting

front 72

does s. enteritids make H2S

back 72

yes

front 73

where would you isolate and ID biochem/serologically from in gastroenteritis

back 73

stool

front 74

where would you isolate and ID biochem/serologically in enteric fevers

back 74

blood

front 75

should you give s. enteritids people antidiarrheals

back 75

no

front 76

how is sepsis of s. enteridis treated

back 76

quinolones and chloramphenicol

front 77

how would you prevent s. enteritidis

back 77

cook poultry, dont eat raw eggs, no reptile pets especially with young kids

front 78

what things do salmonella and edwardsiella have in common

back 78

biochem and epidemiology

front 79

how do arizona ferment lactose

back 79

slowly

front 80

how is arizona acquired

back 80

feces of chicaken, domestic anomals, dried eggs

front 81

what types of disease do arizonas cause

back 81

enterocolitis and enteric fevers also (like salmonella)