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Micro: Streptococci

front 1

what is the first branch of streptococci differentiation based on

back 1

shape (cocci vs. bacilli)

front 2

after shape what is the main differentation of cocci

back 2

ability to grow in air

front 3

after distinguishing which cocci grow in air, what is the differentiation based on between strep and staph

back 3

catalase

positive (staph)
negative (strep)

front 4

after distinguishing staph from strep what is the next step

back 4

bile soluble or optochin sensitive

front 5

is s. pneumo bile solube or optochin sensitive

back 5

yes

front 6

what coccus group grows on bile-esculin agar

back 6

group D strep/enterococcus

front 7

how do you distinguish group D strep

back 7

growth in 6.5% NaCl

front 8

what bacteria grows in 6.5% NaCl

back 8

enterococcus

front 9

what bacteria doesn't grow in 6.5% NaCl

back 9

group D strep

front 10

with respect to lack of growth on bile-esculin agar what is the next differentiative characteristic of streptococcus

back 10

hemolysis pattern on blood agar

front 11

what hemolysis pattern indicates incomplete or partial lysis of RBCs

back 11

alpha/gamma

front 12

what hemolysis pattern indicates complete lysis of RBCs

back 12

beta

front 13

what is the beta hemolytic, bacitracin sensitive strep

back 13

group A

front 14

what is the alpha/gamma hemolytic strep that does not grow on bile-esculin agar

back 14

viridans strep

front 15

what is the bacitracin insensitive strep that is hippurate hydrolyzed or CAMP

back 15

group B

front 16

what is the alpha hemolysis pattern

back 16

green zone around colonies growin on blood agar plate due to incomplete lysis of red blood cells

front 17

what is the beta hemolysis pattern

back 17

clear zone around the colonies due to complete lysis of RBCs

front 18

what is the gamma hemolysis pattern

back 18

no lysis and therefore no change in agar appearance

front 19

what are the indications for different Lancefield groupings

back 19

carbohydrate on cell walls

front 20

what are the main clinical indications of s. pneumo

back 20

respiratory tract infections
meningitis

front 21

what are the two respiratory tract infections from s. pneumo

back 21

pneumonia
bronchitis
otitis media

front 22

what is the most common cause of pneumonia in adults

back 22

s. pneumo

front 23

when does incidence increase in s. pneumo pnemo

back 23

over 40

front 24

what is the s. pnemo pneumonia case fatality rate

back 24

5% but higher in elderly

front 25

how can there be estra pulmonary sites of infection from s. pneumo pneumonia

back 25

bacteremia

front 26

how do the abrupt symptoms usually start in s. pneumo pnsumo

back 26

severe shaking chills

front 27

what are the symptoms of s. pneumo pneumo

back 27

high fever in most
productive cough with rusty sputum
dyspnea, weakness

front 28

what is an uncommon complication with effective antibiotic treatment

back 28

empyema

front 29

how many children will have otitis media at least once by age 6

back 29

75-95%

front 30

how many cases of penumococci account for otitis media

back 30

50%

front 31

what could meningitis result from in s. pneumo pneumo

back 31

bacteremia
sinusitis by direct extension
skull fracture with communication between nasopharynx and subarachnoid space

front 32

how many people will have s. pneumo colonize the upper respiratory tract

back 32

5-50% of population

front 33

how is s. pneumo transmitted

back 33

respiratory droplets (coughing)

front 34

what are the virulence factors of s. pneumo

back 34

polysaccharide capsule
produces IgA protease

front 35

how many antigenic types have been detected of s. pneumo

back 35

85

front 36

what is the role of the polysaccharide capsule

back 36

retards phagocytosis

front 37

what types of infection predispose for s. pneumo lung infection

back 37

viral respiratory infections

front 38

how do viral respiratory infections help s. pneumo to invade the lungs

back 38

mucociliary clearnace

front 39

what type of thing favors the development of bacteremia and sepsis from s. pneumo

back 39

splenectomy or functional splenectomy

front 40

what does s. pneumo look like in the lab

back 40

gram stain smear and culture shows lancet shaped cocci in pairs (diplococci)

front 41

what is the hemolysis pattern of s. pnemo

back 41

alpha hemolytic

front 42

what inhibits s. pnemo

back 42

optochin

front 43

what is the treatment for s pneumo

back 43

penicillin, 3rd gen cephalosporin and second antibiotic (vancomycin) in case of resistance for menintgitis

front 44

what is the mechanism for resistance to penicillin for s. pneumo

back 44

mutation of transpeptidase enzymes (PBP: penicillin binding proteins)

front 45

what are preventive measures of s. pneumo

back 45

23 valent vaccine against capsular polysaccharide for anyone with increased risk of s. pneumo infection and all adults over 65

7 valent conjugated vaccine (pneumococcal polysaccharide and diptheria toxoid) to prevent invasive pneumococcal infections in children

front 46

what are the clinical infections involved with s. pyogenes (group A)

back 46

pharyngitis

other tissues:
impetigo and cellulitis (skin)
erysipelas (skin and subcutaneous tissues)
pneumonia (lung) with empyema

front 47

what is pharyngitis called

back 47

strep throat

front 48

what are the immediate complications of pharyngitis due to s. pyogenes

back 48

peritonsillar abscess, otitis media, mastoiditis

front 49

what are the s. pyogenes sicknesses that are infections plus prominent manifestations of toxins

back 49

scarlet fever
TSS
necrotizing fasciitis

front 50

what are the nonsuppurative diseases of s. pyogenes

back 50

acute glomerulonephritis
rheumatic fever

front 51

what happens in acute glomerulonephritis

back 51

immune complexes bound to glomeruli (type III hypersens)

front 52

who does rheumatic fever usually afflict after sore throat

back 52

6-15 aged children

front 53

what is ARF

back 53

immunologic reaction against host tissue (heart and joint tissue primiarliy) induced by cross-reacting antigens on the streptococci and human tissue

front 54

how long after pharyngitis does ARF affect children

back 54

1-5 weeks

front 55

what usually prevents ARF

back 55

early antibiotic treatment

front 56

what are the "major" criteria for ARF

back 56

carditis
polyarthritis
chorea
erythema marginatum rash
subcutaneous nodules

front 57

what are the "minor" criteria for ARF

back 57

previous rheumatic fever or HD
arthralgia
fever
acute phase reactions (blood test)
prolonged PR interval (ECG)

plus supporting evidence of preceding streptococcal infection (increased ASO, positive throat culture, recent scarlet fever)

front 58

how much of population is genetically predisposed to ARF

back 58

10%

front 59

where does s. pyogenes live

back 59

on throat and skin

front 60

how is s. pyogenes transmitted

back 60

droplets and direct contact

front 61

what are the virulence factors of s. pyogenes

back 61

LTA
streptokinase
M protein
hyaluronidase
C5a peptidase
streptolysins S and O
streptococal pyogenic exotoxins (SPE) types A, B, and C
streptococcal DNase
exotoxin B

front 62

what is LTA

back 62

polymmer of 25 glycerolphosphate subunits boudn to palmitate (lipid)

front 63

what does LTA do

back 63

sticks to fibronectin (a host protein that coats epithelial cells of oropharynx) and allows organism to adhere to epithelial surfaces

front 64

what does streptokinase do

back 64

dissolves clost by activating plasminogen (forms plasmin) which breaks down fibrin in clots

front 65

what is streptokinase used to do

back 65

dissolve unwanted clots like in heart attack patients

front 66

what is the role of M protein

back 66

binds fibrinogen, fibrin, and their degradation products to form a dense coating on the organism's surface

blocks complement deposition

overall: inhibits phagocytossi

front 67

how many different M protein serotypes are there

back 67

80

front 68

what are two important M protein type categories

back 68

rheumatogenic and nephritogenic

front 69

what does hyaluronidase do

back 69

hydrolyzes the ground substance of CT to aid spread

front 70

what does C5a peptidase do

back 70

leaves C5a and destroys its chemotactic signal

front 71

what are streptolysins S and O

back 71

S- oxygen stabile
O- oxygen labile

HEMOLYSINS

front 72

is streptolysin S antigenic

back 72

no

front 73

what is the ASO titer

back 73

antibody vs. streptolysin O, important in diagnosing streptococcal infection

front 74

what can streptolysins be important for

back 74

lysing cells, tissue necrosis

front 75

what are the types of SPE

back 75

a, b, c

front 76

what produces the red rash of scarlet fever

back 76

SPE (erythrogenic toxin)

front 77

what is SPE A associated with

back 77

shock-inducing strep infections (TSS) by stimulation of TNF and IL-1 release

front 78

what is SPE A's TSS similar to

back 78

staphylococcal TSS

front 79

what does streptococcal DNase do

back 79

depolymerizes DNA in necrotic tissues

front 80

is streptococcal DNase antigenic

back 80

yes

front 81

what does exotoxin B do

back 81

protease that rapidly destroys tissue

front 82

what produces exotoxin B

back 82

strains of s. pyogenes that cause necrotizing fasciitis

front 83

what is inolved in the lab diagnosis for s. pyogenes

back 83

gram stained
beta-hemolytic, bacitraicin sensitive
elevated ASO titer (non suppurative)
group determined by antiserum vs. cell wall polysaccharide (quick strep throat test)

front 84

what is the treatment for pyogenes

back 84

penicillin

front 85

is there a vaccine for pyogenes

back 85

nope

front 86

what ar ethe clinical symptoms of s. agalactiae

back 86

endometritis and postpartum sepsis in mother
neonatal meningitis and sepsis in newborn

front 87

how is s. agalactiae (grou B strep) transmitted

back 87

flora of vagina, transmission to newborn at time of birth

front 88

what are the virulence factors of s. sgalactiae

back 88

none ided

front 89

what are the lab findings and diagnosis

back 89

beta hemolysis on blood agar
CAMP positive
hippurate hydrolysis positive
group determined by antiserum against cell wall plysacch

front 90

what is the treatment of s. agalactiae

back 90

penicillin, no vaccine available

front 91

what is the prevention of s. agalactiae

back 91

culture pregnant women and treat mother and newborn if culture positive

front 92

what are the two groups of group D strep

back 92

nonenterococci (s. bovus) and enterococcus

front 93

what is S. bovus normal flora of

back 93

GI and GU tracts

front 94

what is nonenterococcus a rare cause of

back 94

endocarditis and UTI

front 95

what is s. bovus associated with

back 95

colon cancer

front 96

what are members of the enterococcus family

back 96

s. faecalis
enterococcus faecium

front 97

what types of infections are associated with enterococcus

back 97

UTI
endocarditis
wound infections

front 98

why are enterococcus infections difficult to treat

back 98

antibiotic resistance

front 99

how are enterococcal infections often acquired

back 99

nosocomially with weakened host defenses and chronic illness

front 100

how is enterococcus transmitted

back 100

normal flora of the colon
may colonize urethra and female genital tracts

front 101

how can antibiotic resistant enterococcus transmitted

back 101

contact and environmental suraces in hospitals, most from endogenous flora

front 102

what are the toxins and VFs of eneterococcus

back 102

none

low virulence and tends not to cause infections in patients with intact host defenses

front 103

what is involved in the lab diagnosis involved in enterococcus

back 103

grows in 6.5% NaCl and hydrolyzes esculin in presence of 40% bile

front 104

how is enterococcus treated

back 104

penicillin+aminoglycoside
vancomycin

front 105

what type of resistanct is very important to enterococcus

back 105

vancomycin

front 106

what is the resistant move that enterococcus made

back 106

substitute for D-ala-D-ala so it is not in peptidoglycan

front 107

what is anaerobic streptococci called

back 107

peptostreptococcus

front 108

where does peptostreptococcus reside normally

back 108

GI and female genital tract

front 109

what is peptostreptococcus sometimes involved in

back 109

intra-abdominal absecesses

front 110

what is part of the viridans group

back 110

s. sanguis
s. mutans

front 111

what are the clinical manifestations of s. viridans

back 111

endocarditis
miscellaneous (pneumonia, sinusitis, otitis media, bacteremia, dental caries)

front 112

how many of bacterial endocarditis are accounted for by s. viridans

back 112

50%

front 113

what does s. viridans most commonly occur in

back 113

people with abnormal heart valve

front 114

what are the primary symptoms of endocarditis

back 114

fever and heart murmur

front 115

what are secondary manifestations of endocarditis

back 115

septic emboli
cutaneous findinds (Janeway's lesions, Osler's nodes)
glomerulonephritis (immune complex deposition)
CHF, mycotic aneurysms

front 116

what are s. viridans normal flora of

back 116

oropharynx

front 117

how do s. viridans enter the bloodstream

back 117

irritation of oral mucsa, then infect heart valves

front 118

what are the virulence vactors of s. viridans

back 118

some make dextrans that enhance adherence to heart valves and increase risk factor for endocarditis

front 119

how is lab diagnosis for s. viridans made

back 119

alpha/gamma hemolytic on blood agar

many isolates non-groupable by antigenic tests for cell wall carbohydrate antigens

front 120

what is treatment of s. viridans

back 120

penicllin plus aminoglycoside

prophylactic antibiotics for patient with prosthetic heart valves or prior history of endocarditis who undergo dental procedures