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Microbiology Lab midterm review-1,26,3,4,5,7,11,20

front 1

Fixing of bacteria to a slide is done by passing the bacterial smear over the flame of a Bunsen burner BUT it can also be chemically done using

back 1

methanol

front 2

What type of information below cannot be determined by a simple stain?
a. thick/thinness of a cell wall
b. cell arrangement
c. morphology
d. size

back 2

thick/thinness of cell wall

front 3

True or false. When making a smear from solid media you must mix your bacteria in a small amount of water first.

back 3

true

front 4

What is the name of the mordant in Gram-staining called?

back 4

Gram's iodine

front 5

Gram negative cells will show the color of the ____ stain after gram staining

back 5

safranin

front 6

What is the name of the apparatus you use in class to sterilize your inoculating tools?

back 6

Bunsen burner

front 7

There are 2 dilution techniques. Which technique does not allow you to quantify?
a. pour plate
b. streak plate
c. spread plate

back 7

streak plate

front 8

__________ is the most common isolation technique.

back 8

streak plate technique

front 9

After Gram-staining cells appear pink. What does this tell you about the bacterial cells?

back 9

the cells have a thin wall

front 10

The ideal age that a bacterial culture should be used for staining is?

back 10

18-24 hours

front 11

In the spread plate method colonies grow on and in the agar or only on the surface?

back 11

only on the surface

front 12

Turbidity can be used using a machine called a ______________.

back 12

spectrophotometer

front 13

What happens to the % of transmittance as a microbial cell population in nutrient broth increases?

back 13

decreases

front 14

What happens to absorbance as a microbial cell population in nutrient broth increases?

back 14

increases

front 15

In determining bacterial level cells/ml you should choose an agar plate that contains between _________ colonies.

back 15

25-250

front 16

Generation time is the time required for a ___________?

back 16

cell to divide into 2 cells

front 17

In Gram staining the safranin is the __________.

back 17

basic stain

front 18

The shorter the generation time the ________ the growth.

back 18

faster

front 19

A chromophore is the _____________.

back 19

the ion that is colored

front 20

A pure culture is one that?

back 20

only contains a single kind of microbe

front 21

The iris diaphragm can be found _______ the __________.

back 21

below the condenser

front 22

In a Brightfield microscope

back 22

dark objects are viewed against a bright background

front 23

In a parfocal microscope where the specimen has been focused at low power will only need minor adjustment by using the __________ as you increase the magnification.

back 23

fine adjustment knob

front 24

If the total magnification of your specimen is 400x which objective lens was being used?

back 24

high dry lens

front 25

What happens to the field of vision as you change your objective lens from low power to the scanning lens?

back 25

it increases

front 26

Agar is a solidifying agent that is extracted from?

back 26

algae

front 27

Which of the following type of media is used to grow bacteria that require small amounts of oxygen?

back 27

agar deep

front 28

A bacterial organism was introduces into a tube of nutrient broth which has been allowed to sit at 30*C for 24 hours. After this time it is observed to have clumps of microbial cells suspended in the broth while the remainder appears cloudy. What bacterial growth pattern is this exhibiting?

back 28

flocculent

front 29

Which of the following is solid media?
a. nutrient broth
b. Agar slant
c. Agar deep

back 29

agar slant

front 30

Permanent bacteria that can reside and multiply are

back 30

normal microflora

front 31

unwanted microbe

back 31

contaminant

front 32

liquid media is?

back 32

nutrient broth

front 33

cloudy as a result of bacterial growth is?

back 33

turbidity

front 34

intentional introduction of microbes onto or into media

back 34

inoculate

front 35

steam sterilization under pressure

back 35

autoclaving

front 36

population of cells arising from a single bacterial cell

back 36

colony

front 37

to grow

back 37

culture

front 38

substance that turned red when metabolized by bacteria

back 38

tetrazolium chloride

front 39

bacteria that reside temporarily and cannot multiply

back 39

transient

front 40

What tool should be used to introduce microbes into an agar deep?

back 40

inoculating needle