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Summer Immuno Lecture 9

front 1

A developing T cell first learns self-tolerance in which organ?
A. Spleen
B. Lymph node
C. Bone marrow
D. Thymus

back 1

D. Thymus

front 2

The thymic process that teaches developing T cells tolerance to self is called what?
A. Peripheral tolerance
B. Central tolerance induction
C. Clonal selection
D. Affinity maturation

back 2

B. Central tolerance induction

front 3

T-cell precursors enter the thymus after exiting which site?
A. Bone marrow
B. Spleen
C. Peyer patch
D. Lymph node

back 3

A. Bone marrow

front 4

A newly arrived thymocyte lacks CD4, CD8, and TCR. What best describes it?
A. Single-positive
B. Double-positive
C. Receptor-negative immature
D. Fully mature naive

back 4

C. Receptor-negative immature

front 5

After entering the thymus, immature T cells migrate outward to proliferate in which region?
A. Medulla
B. Capsule
C. Hilum
D. Cortex

back 5

D. Cortex

front 6

In the thymic cortex, immature T cells begin gene rearrangement to form which receptor?
A. BCR
B. TCR
C. Fc receptor
D. TLR

back 6

B. TCR

front 7

After successful TCR rearrangement, immature thymocytes begin expressing TCR plus which co-receptors?
A. CD4 and CD8
B. CD28 and B7
C. Fas and FasL
D. PD-1 and PD-L1

back 7

A. CD4 and CD8

front 8

A thymocyte expressing both CD4 and CD8 is called what?
A. Single-positive
B. Double-negative
C. Double-positive
D. Centrally tolerant

back 8

C. Double-positive

front 9

A receptor-negative immature thymocyte is protected from apoptosis by high expression of what?
A. Fas
B. Bcl-2
C. AIRE
D. CCR7

back 9

B. Bcl-2

front 10

Very immature thymocytes express little to no which apoptosis-related molecule?
A. CD4
B. TCR
C. Bcl-2
D. Fas antigen

back 10

D. Fas antigen

front 11

The thymic test for MHC restriction is called what?
A. Positive selection
B. Negative selection
C. Peripheral deletion
D. Somatic hypermutation

back 11

A. Positive selection

front 12

Positive selection is performed mainly by which thymic cells?
A. mTECs
B. FDCs
C. cTECs
D. Tfh cells

back 12

C. cTECs

front 13

Positive selection asks whether a thymocyte’s TCR can recognize what?
A. Foreign antigen
B. Complement fragments
C. B-cell follicles
D. Self-MHC

back 13

D. Self-MHC

front 14

A thymocyte cannot recognize self-MHC during positive selection. What is its fate?
A. Class switching
B. Apoptosis
C. Tfh licensing
D. Germinal rescue

back 14

B. Apoptosis

front 15

cTECs generate some self-peptides for presentation by digesting their own cytoplasm through what process?
A. Degranulation
B. Phagocytosis
C. Autophagy
D. Opsonization

back 15

C. Autophagy

front 16

cTECs can load autophagy-derived peptides onto which molecule?
A. Class II MHC
B. Fc receptor
C. CD40 ligand
D. TCR complex

back 16

A. Class II MHC

front 17

cTECs may also present protein fragments taken from which source?
A. Bloodstream
B. Peyer patch lumen
C. Splenic red pulp
D. Thymus environment

back 17

D. Thymus environment

front 18

During or shortly after positive selection, thymocytes usually become what?
A. Double-negative
B. Single-positive
C. Antibody-secreting
D. Receptor-negative

back 18

B. Single-positive

front 19

After a thymocyte recognizes self-MHC, it begins expressing which chemokine receptor?
A. CCR7
B. CXCR5
C. CXCL13
D. ICOSL

back 19

A. CCR7

front 20

CCR7 expression directs selected thymocytes toward which thymic region?
A. Cortex
B. Capsule
C. Medulla
D. Trabeculae

back 20

C. Medulla

front 21

The medulla attracts CCR7-positive thymocytes because it contains abundant what?
A. AIRE protein
B. CCR7 ligands
C. BCR complexes
D. CD40 ligands

back 21

B. CCR7 ligands

front 22

Negative selection occurs mainly in which thymic region?
A. Cortex
B. Capsule
C. Follicle
D. Medulla

back 22

D. Medulla

front 23

Negative selection asks whether the thymocyte recognizes what?
A. Self-peptides
B. Foreign antibodies
C. Complement fragments
D. Chemokine gradients

back 23

A. Self-peptides

front 24

A thymocyte strongly recognizes self-peptide on MHC. What happens?
A. It becomes CD8
B. It enters blood
C. It is destroyed
D. It becomes Tfh

back 24

C. It is destroyed

front 25

Failure of negative selection can predispose to what?
A. IgA deficiency
B. Autoimmune disease
C. Neutrophil death
D. Complement depletion

back 25

B. Autoimmune disease

front 26

Which medullary cell helps test thymocytes for self-tolerance?
A. cTEC
B. FDC
C. NK cell
D. mTEC

back 26

D. mTEC

front 27

mTECs help delete autoreactive T cells through which process?
A. Positive selection
B. Class switching
C. Negative selection
D. Antigen plucking

back 27

C. Negative selection

front 28

mTECs generate self-peptides partly through what cellular process?
A. Autophagy
B. Degranulation
C. Exocytosis
D. Opsonization

back 28

A. Autophagy

front 29

mTECs express which transcription factor to display tissue-specific antigens?
A. CCR7
B. FasL
C. Bcl-2
D. AIRE

back 29

D. AIRE

front 30

AIRE is best classified as what?
A. Chemokine receptor
B. Transcription factor
C. Death receptor
D. Co-stimulatory ligand

back 30

B. Transcription factor

front 31

AIRE allows mTECs to express antigens normally found where?
A. B-cell follicles
B. Splenic sinusoids
C. Peripheral tissues
D. Bone marrow only

back 31

C. Peripheral tissues

front 32

mTECs use AIRE to express more than how many tissue-specific proteins?
A. One thousand
B. Ten
C. One hundred
D. One million

back 32

A. One thousand

front 33

A child has defective AIRE. Which process is most directly impaired?
A. TCR gene rearrangement
B. Negative selection
C. CD8 lineage choice
D. Cortex proliferation

back 33

B. Negative selection

front 34

A thymocyte fails to bind self-MHC. Which selection step eliminates it?
A. Positive selection
B. Negative selection
C. AICD contraction
D. Tfh licensing

back 34

A. Positive selection

front 35

A thymocyte binds self-peptide too strongly. Which selection step eliminates it?
A. Somatic hypermutation
B. Positive selection
C. Negative selection
D. Germinal rescue

back 35

C. Negative selection

front 36

Which thymic cell type is most associated with AIRE expression?
A. cTEC
B. mTEC
C. FDC
D. Tfh cell

back 36

B. mTEC

front 37

Which thymic cell type is most associated with testing MHC restriction?
A. mTEC
B. Tfh cell
C. cTEC
D. FDC

back 37

C. cTEC

front 38

What is the purpose of central tolerance induction?
A. Increase antibody affinity
B. Activate naive B cells
C. Prevent self-reactive T cells
D. Recruit neutrophils

back 38

C. Prevent self-reactive T cells

front 39

Why is AIRE important for central tolerance?
A. It binds CD8 directly
B. It creates CCR7 ligands
C. It prevents TCR rearrangement
D. It displays peripheral antigens

back 39

D. It displays peripheral antigens

front 40

The thymocyte migration sequence is best summarized as which pathway?
A. Medulla to cortex
B. Cortex to medulla
C. Spleen to thymus
D. Follicle to PALS

back 40

B. Cortex to medulla

front 41

A second thymic cell type helps test developing T cells for self-tolerance in the medulla. What is this cell called?
A. Follicular dendritic cell
B. Thymic dendritic cell
C. Cortical epithelial cell
D. Marginal-zone B cell

back 41

B. Thymic dendritic cell

front 42

Thymic dendritic cells are abbreviated as what?
A. FDC
B. cTEC
C. TDC
D. Tfh

back 42

C. TDC

front 43

Thymic dendritic cells contribute to tolerance testing by presenting what?
A. Foreign antigens only
B. Self-antigens
C. Complement fragments
D. Antibody Fc regions

back 43

B. Self-antigens

front 44

TDCs present self-antigens on which molecules?
A. MHC molecules
B. BCR complexes
C. Fc receptors
D. Chemokine receptors

back 44

A. MHC molecules

front 45

In addition to making self-antigens, TDCs also receive self-antigens from which cells?
A. cTECs
B. mTECs
C. FDCs
D. Tfh cells

back 45

B. mTECs

front 46

Medullary thymic dendritic cells are resident cells of which region?
A. Thymic cortex
B. Splenic PALS
C. Thymic medulla
D. Lymph-node cortex

back 46

C. Thymic medulla

front 47

Medullary TDCs develop from which precursor source?
A. Bone-marrow-derived precursors
B. Thymic epithelial precursors
C. Splenic stromal precursors
D. Germinal-center precursors

back 47

A. Bone-marrow-derived precursors

front 48

The final thymic output should recognize which structure?
A. Free self-antigen
B. Complement-opsonized antigen
C. Self MHC-peptide complex
D. Antibody constant region

back 48

C. Self MHC-peptide complex

front 49

Mature thymic graduates should not recognize self-antigens presented by which cells?
A. B cells and FDCs
B. TDCs and mTECs
C. NK cells and CTLs
D. cTECs and neutrophils

back 49

B. TDCs and mTECs

front 50

A T cell exits the thymus after testing. Which receptor profile is desired?
A. Binds self-antigen strongly
B. Ignores all MHC molecules
C. Recognizes self MHC-peptide
D. Recognizes antibody Fc regions

back 50

C. Recognizes self MHC-peptide

front 51

A young thymus tests approximately how many double-positive cells daily?
A. 2 million
B. 6 million
C. 60 million
D. 600 million

back 51

C. 60 million

front 52

Of the daily double-positive thymocytes tested, approximately how many single-positive cells exit?
A. 20 million
B. 2 million
C. 60 million
D. 600,000

back 52

B. 2 million

front 53

Most double-positive thymocytes tested daily undergo what fate?
A. Clonal expansion
B. Class switching
C. Somatic hypermutation
D. Apoptosis

back 53

D. Apoptosis

front 54

Roughly what percentage of tested thymocytes mature and exit daily?
A. 30%
B. 10%
C. 3%
D. 90%

back 54

C. 3%

front 55

The Goldilocks hypothesis addresses how one TCR can signal which outcomes?
A. Switching, mutation, apoptosis
B. Selection, deletion, activation
C. Opsonization, lysis, chemotaxis
D. Trafficking, adhesion, rolling

back 55

B. Selection, deletion, activation

front 56

According to the Goldilocks hypothesis, why do so few thymocytes mature?
A. Most cannot signal correctly
B. Most lack bone-marrow origin
C. Most become plasma cells
D. Most migrate into spleen

back 56

A. Most cannot signal correctly

front 57

A T cell survives thymic testing because its TCR interaction strength is “just right.” What does this imply?
A. Strong self-antigen binding
B. No MHC recognition
C. Balanced self-MHC recognition
D. Direct antibody binding

back 57

C. Balanced self-MHC recognition

front 58

During positive selection, surviving T cells should have what type of interaction with self MHC-peptide?
A. Relatively weak
B. Extremely strong
C. Completely absent
D. Antibody-mediated

back 58

A. Relatively weak

front 59

Why must self MHC-peptide interaction be relatively weak?
A. Allows focus on antigen
B. Prevents MHC expression
C. Blocks APC activation
D. Eliminates CD4 cells

back 59

A. Allows focus on antigen

front 60

In the Goldilocks model, interaction with self-peptide presented by medullary TDCs should be what?
A. Very strong
B. Very weak
C. Completely absent
D. Antibody dependent

back 60

B. Very weak

front 61

A thymocyte binds self-peptide on medullary TDCs too strongly. What happens?
A. It becomes nTreg always
B. It is killed
C. It exits rapidly
D. It becomes CD8 only

back 61

B. It is killed

front 62

A thymocyte’s interaction with self-peptide is weak enough to avoid deletion but sufficient for later APC interaction. What does this represent?
A. Goldilocks signaling
B. BCR crosslinking
C. Fc receptor binding
D. Complement activation

back 62

A. Goldilocks signaling

front 63

Even after thymic exit, many T cells die because they fail to find what?
A. B7 proteins
B. Cognate antigen
C. Fc regions
D. Complement fragments

back 63

B. Cognate antigen

front 64

If a naive T cell never finds cognate antigen, it dies after approximately how long?
A. 2 days
B. 1 week
C. 6 weeks
D. 6 months

back 64

C. 6 weeks

front 65

Some T cells pass negative selection despite being self-reactive because their antigen is what?
A. Very common
B. Very rare
C. Bacterial only
D. Antibody-bound

back 65

B. Very rare

front 66

Escaped self-reactive T cells often fail to cause disease because their cognate antigen is unlikely to be found where?
A. Bone marrow
B. Secondary lymphoid organs
C. Thymic cortex
D. Blood plasma

back 66

B. Secondary lymphoid organs

front 67

A T cell escapes deletion because its rare tissue-specific antigen was not encountered. What is this cell?
A. Self-reactive T cell
B. Marginal-zone B cell
C. Follicular helper cell
D. Thymic dendritic cell

back 67

A. Self-reactive T cell

front 68

Rare tissue-specific cognate antigens may allow some T cells to pass which process?
A. Positive selection
B. Negative selection
C. Class switching
D. Somatic hypermutation

back 68

B. Negative selection

front 69

In the thymic medulla, a subset of CD4 T cells becomes which regulatory population?
A. iTregs
B. Tfh cells
C. nTregs
D. CTLs

back 69

C. nTregs

front 70

Natural regulatory T cells are selected mainly in which location?
A. Thymic cortex
B. Thymic medulla
C. Splenic red pulp
D. Lymph-node medulla

back 70

B. Thymic medulla

front 71

nTregs arise from which broad T-cell type?
A. CD4 T cells
B. CD8 T cells
C. NK cells
D. B cells

back 71

A. CD4 T cells

front 72

T cells with weak affinity for self-antigens are generally selected to become what?
A. Th cells
B. nTregs
C. Plasma cells
D. Macrophages

back 72

A. Th cells

front 73

T cells with too strong affinity for self-antigens are usually what?
A. Expanded
B. Destroyed
C. Class-switched
D. Exported

back 73

B. Destroyed

front 74

T cells with intermediate affinity for self-antigens are selected to become what?
A. Th17 cells
B. CTLs
C. nTregs
D. FDCs

back 74

C. nTregs

front 75

nTreg-selected cells are induced to express which gene?
A. AIRE
B. Bcl-2
C. GlyCAM-1
D. Foxp3

back 75

D. Foxp3

front 76

Foxp3 expression is associated with which regulatory lineage?
A. Regulatory T cells
B. Marginal-zone B cells
C. Thymic dendritic cells
D. Cortical epithelial cells

back 76

A. Regulatory T cells

front 77

nTregs become activated when they encounter which antigen type?
A. Self-antigen
B. Bacterial LPS
C. Viral dsRNA
D. Antibody Fc

back 77

A. Self-antigen

front 78

Once activated, nTregs suppress which cells?
A. Plasma B cells
B. Potentially self-reactive T cells
C. Resident macrophages only
D. Follicular dendritic cells

back 78

B. Potentially self-reactive T cells

front 79

The main protective role of nTregs is preventing damage from which cells?
A. Self-reactive T cells
B. Overactive neutrophils
C. IgE-producing B cells
D. Antigen-presenting macrophages

back 79

A. Self-reactive T cells

front 80

Both nTregs and iTregs express which protein?
A. CTLA-4
B. AIRE
C. Foxp3
D. CD40L

back 80

C. Foxp3

front 81

Which regulatory T-cell type protects mainly against self-reactive T cells?
A. iTregs
B. nTregs
C. Th0 cells
D. Tfh cells

back 81

B. nTregs

front 82

Which regulatory T-cell type restrains overreaction to foreign invaders?
A. nTregs
B. CTLs
C. Tfh cells
D. iTregs

back 82

D. iTregs

front 83

nTregs and iTregs both express Foxp3 but differ mainly in what?
A. Target of suppression
B. MHC class restriction
C. TCR gene number
D. Bone marrow origin

back 83

A. Target of suppression

front 84

Which regulatory cell is naturally selected within the thymus?
A. iTreg
B. Th17
C. nTreg
D. Tfh

back 84

C. nTreg

front 85

Which regulatory cell is inducible from naive Th cells in peripheral settings?
A. nTreg
B. iTreg
C. CTL
D. TDC

back 85

B. iTreg

front 86

A potentially self-reactive virgin T cell leaves normal circulation and enters kidney tissue. Which safety mechanism protects against autoimmunity here?
A. Central tolerance induction
B. Peripheral tolerance induction
C. Somatic hypermutation
D. Missing-self recognition

back 86

B. Peripheral tolerance induction

front 87

A virgin T cell recognizes cognate antigen on a nonprofessional tissue cell but receives no second signal. What happens?
A. It becomes anergic
B. It undergoes class switching
C. It becomes a Tfh cell
D. It activates macrophages

back 87

B. It undergoes class switching

front 88

A virgin T cell recognizes antigen on a normal kidney cell but does not activate. What signal is missing?
A. Complement fixation
B. TCR rearrangement
C. Co-stimulation
D. Fc binding

back 88

C. Co-stimulation

front 89

Why does a normal kidney cell usually fail to activate a virgin T cell?
A. It lacks B7
B. It lacks MHC I
C. It secretes IgA
D. It expresses Foxp3

back 89

A. It lacks B7

front 90

A virgin T cell becomes anergized after recognizing antigen without co-stimulation. What is its eventual fate?
A. Class switching
B. NK licensing
C. Death
D. Memory formation

back 90

C. Death

front 91

A rare self-reactive virgin T cell receives such strong antigen crosslinking that co-stimulation is bypassed. Which backup mechanism limits it?
A. Receptor editing
B. Missing-self recognition
C. Activation-induced cell death
D. Somatic hypermutation

back 91

C. Activation-induced cell death

front 92

Instead of testing every single T cell perfectly, the immune system uses at least how many tolerance mechanisms?
A. Two
B. Three
C. Four
D. Five

back 92

D. Five

front 93

Most B cells are tolerized in which location?
A. Thymus
B. Bone marrow
C. Spleen
D. Lymph node

back 93

B. Bone marrow

front 94

A developing B cell recognizes self-antigen in the bone marrow. What process gives it another chance?
A. Receptor editing
B. Positive selection
C. AICD
D. Missing-self recognition

back 94

A. Receptor editing

front 95

Receptor editing most directly involves rearrangement of which B-cell component?
A. Heavy chain
B. Fc region
C. J chain
D. Light chain

back 95

D. Light chain

front 96

A germinal-center B cell undergoes somatic hypermutation and develops self-reactivity. Why is activation by FDCs unlikely?
A. Self-antigens lack MHC
B. Self-antigens usually aren't opsonized
C. FDCs lack antigen receptors
D. FDCs only bind T cells

back 96

B. Self-antigens usually aren't opsonized

front 97

A self-reactive B cell arises after somatic hypermutation. Why is T-cell help unlikely?
A. Matching Th cells are absent
B. B cells cannot present antigen
C. CD40 is always absent
D. Th cells lack MHC II

back 97

A. Matching Th cells are absent

front 98

A virus-infected cell downregulates Class I MHC to evade CTLs. Which immune cell can kill it?
A. Th2 cell
B. Plasma cell
C. NK cell
D. B cell

back 98

C. NK cell

front 99

NK-cell killing of cells with reduced Class I MHC is called what?
A. Receptor editing
B. Peripheral tolerance
C. Central tolerance
D. Missing-self recognition

back 99

D. Missing-self recognition

front 100

In missing-self recognition, NK cells kill because they fail to receive what signal?
A. Co-stimulatory signal
B. Inhibitory signal
C. Chemokine signal
D. TCR signal

back 100

B. Inhibitory signal

front 101

The inhibitory signal for NK cells depends on recognition of which host molecule?
A. Class I MHC
B. Class II MHC
C. CD40 ligand
D. Antibody Fc

back 101

A. Class I MHC

front 102

An NK cell cannot recognize the body’s Class I MHC. Without licensing, what risk would this create?
A. Autoimmune NK killing
B. Failed BCR editing
C. Excess IgE switching
D. Thymic T-cell deletion

back 102

A. Autoimmune NK killing

front 103

NK cells must be licensed through which screening process?
A. Negative selection
B. Receptor editing
C. Positive selection
D. Class switching

back 103

C. Positive selection

front 104

During NK-cell licensing, functional NK cells must bind what?
A. Foreign antigen
B. Self-MHC
C. Complement fragments
D. B7 proteins

back 104

B. Self-MHC

front 105

NK cells that cannot bind self-MHC during licensing become what?
A. Anergized
B. Activated
C. Class-switched
D. Opsonized

back 105

A. Anergized

front 106

Which scenario best illustrates peripheral tolerance induction?
A. T cell deleted in thymus
B. B cell edits light chain
C. T cell sees antigen, no B7
D. NK cell detects missing MHC

back 106

C. T cell sees antigen, no B7

front 107

Which scenario best illustrates receptor editing?
A. Self-reactive B cell rearranges light chain
B. CTL dies after repeated activation
C. NK cell binds self-MHC
D. Th cell becomes Tfh

back 107

A. Self-reactive B cell rearranges light chain

front 108

Which scenario best illustrates missing-self recognition?
A. T cell lacks co-stimulation
B. B cell lacks Th help
C. NK cell senses low MHC I
D. mTEC expresses AIRE

back 108

C. NK cell senses low MHC I

front 109

A self-reactive B cell after somatic hypermutation usually fails because it cannot efficiently get which two supports?
A. FDC antigen and Th help
B. CTL killing and FasL
C. NK licensing and IL-2
D. AIRE and TDC antigen

back 109

A. FDC antigen and Th help

front 110

Which tolerance mechanism is specific to B cells in this material?
A. Peripheral anergy
B. Receptor editing
C. Missing-self recognition
D. Activation-induced death

back 110

B. Receptor editing