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Lecture 15

front 1

What are the six categories of antibody effector functions?

back 1

Neutralization, opsonization, complement activation, ADCC, mast cell degranulation, agglutination.

front 2

What is neutralization?

back 2

Antibodies block pathogens or toxins from entering cells.

front 3

What is opsonization?

back 3

Antibodies coat pathogens to enhance phagocytosis.

front 4

What is complement activation?

back 4

IgM or IgG trigger complement leading to pathogen lysis.

front 5

What is ADCC?

back 5

NK cells kill antibody-coated target cells.

front 6

What is mast cell degranulation?

back 6

IgE triggers release of histamine for allergy and parasite defense.

front 7

What is agglutination?

back 7

Antibodies clump pathogens together for easier clearance.

front 8

What are the characteristics of IgM?

back 8

First responder, pentamer, strong complement activation, good at agglutination.

front 9

What are the characteristics of IgE?

back 9

Involved in allergies and parasites, binds mast cells, low concentration in serum.

front 10

What are the characteristics of IgD?

back 10

Found on naïve B cells, functions mainly as a B cell receptor.

front 11

What are the characteristics of IgA?

back 11

Mucosal immunity, dimer, found in secretions like saliva and lungs.

front 12

What are the characteristics of IgG?

back 12

Main serum antibody, long half-life, crosses placenta, involved in opsonization and ADCC.

front 13

What are Fc receptors?

back 13

Receptors that bind the Fc region of antibodies and trigger immune responses.

front 14

What do Fc receptors do?

back 14

Translate antibody binding into cellular responses like phagocytosis or killing.

front 15

What are ITAMs?

back 15

Activating signaling motifs that trigger immune cell activation.

front 16

What are ITIMs?

back 16

Inhibitory signaling motifs that suppress immune activation.

front 17

Which overrides: ITAM or ITIM?

back 17

ITIM overrides ITAM (inhibitory signals dominate).

front 18

What is affinity?

back 18

Strength of binding between receptor and antigen-MHC.

front 19

What is FcγR?

back 19

Fc receptor that binds IgG and mediates phagocytosis and ADCC.

front 20

What is FcεR?

back 20

Fc receptor that binds IgE and triggers mast cell degranulation.

front 21

What is FcαR?

back 21

Fc receptor that binds IgA and supports mucosal defense and phagocytosis.

front 22

What is FcμR?

back 22

Fc receptor that binds IgM and regulates IgM responses.

front 23

What is FcRn?

back 23

Neonatal Fc receptor that binds IgG and protects it from degradation.

front 24

What are the two functions of FcRn?

back 24

Protect IgG from degradation and transfer IgG to fetus.

front 25

What is IgG recycling via FcRn?

back 25

IgG is taken into cells, bound by FcRn in endosomes, protected from degradation, and released back into circulation.

front 26

What is the significance of FcRn recycling?

back 26

Extends IgG half-life (~21 days) and supports long-term immunity.

front 27

What is pIgR?

back 27

Poly-Ig receptor that transports antibodies across epithelial cells.

front 28

Which antibodies use pIgR?

back 28

IgA and IgM.

front 29

What is the role of the J chain?

back 29

Links antibody units together and is required for transport via pIgR.

front 30

What is the function of pIgR?

back 30

Moves IgA and IgM to mucosal surfaces.

front 31

How is pIgR different from FcRn?

back 31

pIgR exports antibodies to mucosa, FcRn protects antibodies in circulation.

front 32

How is antibody secretion turned off?

back 32

Through negative feedback mechanisms.

front 33

What is FcγRIIB?

back 33

An inhibitory Fc receptor on B cells that binds IgG-antigen complexes.

front 34

What is the role of FcγRIIB?

back 34

Provides negative feedback to reduce antibody production.

front 35

How do ITIMs function in FcγRIIB?

back 35

They send inhibitory signals to suppress B cell activation.

front 36

What is feedback inhibition in B cells?

back 36

Increased IgG leads to increased inhibition of further antibody production.

front 37

What are therapeutic antibodies?

back 37

Lab-made antibodies used for treatment of diseases.

front 38

What is a mouse antibody?

back 38

Fully mouse-derived, highly immunogenic.

front 39

What is a chimeric antibody?

back 39

Mouse variable region with human constant region.

front 40

What is a humanized antibody?

back 40

Mostly human with only mouse CDR regions.

front 41

What is a fully human antibody?

back 41

Entirely human, least immunogenic.

front 42

What is Rituximab?

back 42

A chimeric antibody targeting CD20 to kill B cells, used for lymphoma and autoimmune diseases.

front 43

What is Adalimumab?

back 43

A fully human antibody targeting TNF-alpha to reduce inflammation in autoimmune diseases.

front 44

What is Ipilimumab?

back 44

A fully human antibody targeting CTLA-4 to enhance T cell activation in cancer therapy.

front 45

What are the general steps of CD8⁺ T cell activation?

back 45

Recognition of antigen on MHC I, co-stimulation, cytokine signaling, clonal expansion, differentiation.

front 46

What are the three signals for CD8⁺ T cell activation?

back 46

TCR + MHC I, CD28-B7 costimulation, cytokines (IL-2).

front 47

What is the sequential model of CD8 activation?

back 47

CD4 T cells activate first, then help activate CD8 T cells.

front 48

What is the simultaneous model?

back 48

CD4 and CD8 T cells are activated at the same time by the same APC.

front 49

Which model is better supported?

back 49

Simultaneous model.

front 50

What is dendritic cell licensing?

back 50

CD4 T cells activate dendritic cells to better stimulate CD8 T cells.

front 51

What is the role of CD40

back 51

CD40L? / CD40L on CD4 T cells binds CD40 on dendritic cells to activate them.

front 52

What is cross-presentation?

back 52

Dendritic cells present external antigens on MHC I to activate CD8 T cells.

front 53

What is the role of IL-2?

back 53

Promotes T cell growth and proliferation.

front 54

Which cells produce IL-2?

back 54

Mainly CD4 T cells.

front 55

How do cytotoxic T lymphocytes (CTLs) kill target cells?

back 55

Using perforin/granzymes and Fas–FasL pathways.

front 56

What is perforin?

back 56

Protein that forms pores in target cell membranes.

front 57

What are granzymes?

back 57

Enzymes that enter cells and trigger apoptosis.

front 58

What is the Fas–FasL pathway?

back 58

FasL on T cells binds Fas on target cells to induce apoptosis.

front 59

Why are adhesion molecules important?

back 59

They stabilize interaction between CTL and target cell.

front 60

What is IFN-γ?

back 60

Cytokine that activates macrophages and has antiviral effects.

front 61

What is lymphotoxin (LT)?

back 61

Cytokine involved in inflammation and cell killing.

front 62

What are NK cells?

back 62

Innate lymphocytes that kill infected or cancer cells.

front 63

What are characteristics of NK cells?

back 63

No antigen-specific receptors, rapid response, detect abnormal cells.

front 64

What lineage are NK cells from?

back 64

Lymphoid lineage.

front 65

What cytokines activate NK cells?

back 65

IL-12, IL-15, IL-2.

front 66

What cytokine do NK cells secrete?

back 66

IFN-γ.

front 67

How do NK cells decide to kill?

back 67

Balance between activating and inhibitory signals.

front 68

What is the missing self model?

back 68

NK cells kill cells lacking MHC I.

front 69

What is the balanced signals model?

back 69

NK cell activation depends on net activating vs inhibitory signals.

front 70

Which NK model is more accurate?

back 70

Balanced signals model.

front 71

What are inhibitory NK ligands?

back 71

MHC class I molecules.

front 72

What are activating NK ligands?

back 72

Stress-induced molecules on infected or tumor cells.

front 73

What is ADCC in NK cells?

back 73

NK cells bind IgG-coated targets and kill them.