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Lecture 8: The MHC and Antigen Presentation 1

front 1

What are the 3 functions of the innate immune system?

back 1

Physical/chemical barriers, cellular responses, activation of adaptive immunity

front 2

Why is MHC necessary?

back 2

T cells can’t recognize free antigen → need MHC to present peptide→ enables T cell activation

front 3

Similarities between BCR and TCR?

back 3

Both are antigen-specific and generated by gene rearrangement

front 4

Differences between BCR and TCR?

back 4

BCR binds free antigen (no MHC); TCR requires processed antigen + MHC

front 5

What is self-MHC restriction?

back 5

T cells only recognize antigen on self-MHC

front 6

Where are MHC molecules expressed?

back 6

Most cells (Class I), APCs (Class II)

front 7

Where are TCRs expressed?

back 7

Only on T cells

front 8

Components of MHC Class I?

back 8

α chain + β2-microglobulin

front 9

Components of MHC Class II?

back 9

α chain + β chain

front 10

Similarity between Class I & II?

back 10

Both have peptide-binding groove and present antigens to T cells

front 11

Key difference between Class 1 and 2?

back 11

Class 1 = 1 chain + β2m; Class 2 = 2 chains

front 12

Where is MHC Class I found?

back 12

All nucleated cells

front 13

Where is MHC Class II found?

back 13

APCs (Dendritic cells, macrophages, B cells)

front 14

What is the binding groove?

back 14

Site where peptide binds on MHC

front 15

Why is the binding groove important?

back 15

Determines which peptides can be presented

front 16

Steps of antigen presentation?

back 16

Breakdown → load on MHC → move to surface → T cell recognition

front 17

What is the central dogma?

back 17

DNA → RNA → Protein

front 18

What is an Allele?

back 18

Different versions of a gene

front 19

What is an Allotype?

back 19

Protein product of allele

front 20

What is a SNP?

back 20

a polymorphism of a single nucleotide (not quite same as mutation)

front 21

What is Co-dominance?

back 21

Both alleles expressed equally

front 22

What is a Haplotype?

back 22

Linked genes inherited together

front 23

What is a Syngeneic Haplotype?

back 23

Genetically identical

front 24

What is Congenic Haplotype?

back 24

Identical except one locus

front 25

What is Polygeny?

back 25

Multiple genes → one protein

front 26

What is a Polymorphism?

back 26

Many versions of a protein due to multiple alleles

front 27

Where are MHC genes located?

back 27

Chromosome 6

front 28

What is HLA?

back 28

Human MHC known as Human Leukocyte Antigen complex

front 29

What does Complex I encode?

back 29

HLA-A, B, C

front 30

What does Complex II encode?

back 30

HLA-DP, DQ, DR

front 31

What does Complex III encode?

back 31

Complement + inflammatory cytokines

front 32

Complex I presents to which cells?

back 32

CD8 T cells

front 33

Complex II presents to which cells?

back 33

CD4 T cells

front 34

Why is co-dominance important?

back 34

Increases antigen presentation diversity

front 35

How are MHC genes inherited?

back 35

As haplotypes from each parent and expressed co-dominantly

front 36

What is MHC diversity?

back 36

Many alleles in population

front 37

Why is MHC diversity important?

back 37

Protects against many pathogens

front 38

What is MHC promiscuity?

back 38

One MHC binds many peptides

front 39

Heterozygote advantage?

back 39

More alleles = better protection

front 40

Balancing selection?

back 40

natural selection that maintains multiple alleles in a population keeping MHC diversity high

front 41

Directional selection?

back 41

Favors one allele, reducing diversity

front 42

What are anchor residues?

back 42

Key amino acids that bind MHC

front 43

Why are anchor residues important?

back 43

to determine which peptides fit in the groove

front 44

Anchor residues are more important in which class?

back 44

Class I because of more restrictive binding

front 45

MHC are related to promiscuity?

back 45

They relate to promiscuity because Class 2 have less strict binding making them more promiscuous.

front 46

How does MHC activate T cells?

back 46

Signal 1 = MHC + peptide → TCR

front 47

What else is needed for T cells activation?

back 47

Signal 2 (costimulation) + Signal 3 (cytokines)

front 48

What are the 3 signals for T cell activation?

back 48

MHC + peptide (Signal 1), Costimulation (Signal 2) and Cytokines (Signal 3)