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campbell 19 viruses

front 1

General Virology & Tobacco Mosaic Disease

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no data

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virus

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smaller and simpler than eukaryotes and prokaryotes infectious particle consisting of little more than genes packaged in a protein coat cant reproduce, cant do metabolic actives outside of host virus latin root = poison obligate, intracellular parasite: can only replicate within a host cell

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tobacco mosaic disease

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stunts the growth of tobacco plants gives tobacco plants mottled, mosaic, coloration Adolf Mayer transmittable by rubbing bad to good particle that causes = tobacco mosaic virus TMV

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structure of viruses viruses [] consisting of [] enclosed by [___]

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Answer: infectious particle; one or more nucleic acid; protein coat and for some membranous envelope

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Genomes and Capsids

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no data

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virus genome

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can be double stranded dna (norm), single stranded dna, double strand rna, or single strand rna depending on virus

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capsid

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protein shell enclosing the viral genome rod-shaped, polyhedral, or more complex helical viruses icosahedral viruses built from capsomeres

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helical

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rod-shaped viruses, eg TMV rod shaped made of protein arranged in helix

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icosahedral viruses

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polyhedral capsid that has 20 triangular facets

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capsomeres

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proteins that make up capsids, many of them make up capsids

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bacteriophages

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most complex capsid Infect e. coli 7 types

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viral envelopes

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derived from membranes of host cell contain host cell phospholipids and membrane proteins contain proteins + glycoproteins of viral origin an accessory structure that helps viruses infect hosts

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Viral Replication & Phages

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no data

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host range

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viruses can only infect a limited number of host species. name of the range

front 15

the lytic cycle

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the pages replicative cycle that culminates in death of the host cell lyric = last stage of infection, when bacterium LYSES (breaks open) phages released that were produced within cell then phages can infect a healthy cell so a bacteria population can be destroyed a phage that only replicates in this cycle is virulent

front 16

virulent phage

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T4, a phage that replicates only by a lytic cycle

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lysogeny

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state when phages [___] with host cells, prophages can generate active phages that lyse cells Answer: COEXIST

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the lysogenic cycle

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allows replication of phage w/o killing host temperate phages

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temperate phages

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lambda, phage that can go through both lysogenic and lytic cycles to replicate

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prophage

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viral DNA integrated into the host cell's chromosome, lysogenic cycle

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Bacterial Defenses

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no data

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bacterial defenses

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natural selection favors bacteria w/ surface protein no longer recognized as receptor by particular phage when phage dna enters cell, foreign, so restriction enzyme cuts up

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restriction enzyme

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cellular enzyme that restricts phage ability to replicate in bacterium

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bacterium dna

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bacterium dna is methylated so that restriction enzyme doesnt attack own dna

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palindrome

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dna sequence reading same backwards and forwards

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crispr

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clustered regularly interspaced short palindromic repeats associated w/ a phage that had infected cell

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cas

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crispr-associate proteins, identify and cut phage dna (defending cell from phage); uses portion of phage related rna to identify invading phage dna and cut it

front 28

Animal Viruses & Retroviruses

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no data

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viral envelopes

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virus uses envelope to enter host cell

front 30

replicative cycle of rna genome virus

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glycoproteins on viral envelope bind to receptor molecules on host cell capsid and viral genome enter cell capsid digested by cellular enzyme, viral genome released viral genome functions as template for synthesis of complementary RNA by viral RNA polymerase copies of rna are made using complementary rna strands; complementary rna also mrna, so complementary rna translated to capsid proteins + glycoproteins for viral envelope (in ER and Golgi apparatus) vesicles transport envelope glycoproteins to plasma membrane capsid assembles around viral genome molecule new virus buds from the cell, envelope studded with glycoproteins embedded in membrane (from host cell)

front 31

herpesvirus

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envelope not derived from plasma membrane temporary cloacked in nuclear envelope derived envelope then shed and new golgi apparatus envelope double stranded dna genome that replicate in host dna

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retroviruses

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RNA animal viruses with the most complicated replicative cycles retroviruses have an enzyme called [___] that transcribes and RNA remplace into a dna copy Answer: reverse transcriptase

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HIV

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human immunodeficiency virus, causes AIDS

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AIDS

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acquired immunodeficiency syndrome, caused by HIV virus

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HIV cycle

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1.) enters host cell 2.) reverse transcriptase molecules released into cytoplasm 3.) dna is made 4.) new DNA enters cell nucleus 5.) dna integrates into dna of a chromosome 6.) rna polymerase makes viral rna from this viral dna and viral proteins, new viruses released from cell

front 36

provirus

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integrated viral dna that never leaves the host genome, permanent resident of the cell

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Evolution & Medical Treatments

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no data

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evolution of viruses

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possible original source of viral genomes plasmids and transposons

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plasmid

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small circular dna molecules found in bacteria and unicellular fungi called yeasts; exist apart from genome, replicate independently of genome. occasionally transferred between cells

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transposons

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dna segments that can move from one location to another withing a cell's genome

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mobile genetic element

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feature describing plasmids, transposons, and viruses

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vaccine

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harmless derivative of a pathogen stimulating the immune system to mount defenses against harmful pathogen, taken BEFORE virus affects

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antibiotics

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help us recover from bacterial infection, powerless against virus, they kill bacteria by inhibiting enzymes specific to bacteria but have little or no effect on eukaryotic/viral encoded enzymes

front 44

acyclovir

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impeded herpes virus replication; inhibits the viral polyamerase duplicating viral dna but not eukaryotic one

front 45

azydothymidine (AZT)

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curbs HIV replication, interfering with reverse transcriptase

front 46

maraviroc

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blocks protein on human immune cell binding to HIV virus

front 47

Emerging Viruses & Plants

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no data

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emerging virus

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viruses suddenly become apparent (e.g. HIV)

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epidemic

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a widespread outbreak (e.g. ebola, an emerging virus causing hemorragic fever; zika; chikungunya); occur when genetic changes allow a new viral strain to be easily transmitted between humans

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pandemic

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a global epidemic

front 51

recombination in spread

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how can recombination cause viruses to spread rapidly? for example, in pigs. h1n1 rna strands are made up of 9 rna segments, which can mix and match, reassort, during ciral assembly, creating multiple genetic variations and combinations. when they reassort, they might acquire the ability to spread to humans

front 52

horizontal transmission

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an external source infects the plant. invading virus must get past epidermis of plant, plant becomes susceptible if external injury

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vertical transmission

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plant inherits from parent

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plasmodesmata

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the cytoplasmic connections that penetrate the walls between adjacent plant cells

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Prions

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no data

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prions

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infectious proteins, cause degenerative brain diseases in animal species misfolded form of a protein normally in brain cells scrapie in sheep, mad cow diseasem jakob disease in humans transmitted in food (mad cow disease, cannibalism of a prion host) long incubation (long time to show up) molecular basis, gets into cell containing normal form of protein, misfolds normal protein into prion alzheimers and parkinsons involvement