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83 notecards = 21 pages (4 cards per page)

Viewing:

immunology lecture 19 key points

front 1

what is hypersensitivity?

back 1

inappropriate secondary responses against harmless antigens

result in more harm than benefit (the reaction is worse than the antigen)

front 2

types of classification of hypersensitivity?

back 2

time until response

mechanisms

types of damage caused

front 3

type I hypersensitivity mechanism

back 3

IgE

front 4

type I hypersensitivity allergy

back 4

peanuts

front 5

type II hypersensitivity mechanism

back 5

IgG/IgM (cells)

front 6

type II hypersensitivity allergy

back 6

hemolytic anemia

front 7

type II hypersensitivity transplantation

back 7

hyperacute rejection

front 8

type II hypersensitivity autoimmunity

back 8

autoimmune hemolytic anemia

front 9

type III hypersensitivity mechanism

back 9

IgG/IgM (IC)

front 10

type III hypersensitivity allergy

back 10

serum sickness

front 11

type III hypersensitivity transplantation

back 11

chronic rejection

front 12

type III hypersensitivity autoimmunity

back 12

SLE

front 13

type IV hypersensitivity mechanism

back 13

T cells

front 14

type IV hypersensitivity allergy

back 14

poison ivy

front 15

type IV hypersensitivity transplantation

back 15

acute rejection

front 16

type IV hypersensitivity autoimmunity

back 16

type I diabetes

front 17

which characteristics make parasites different than other pathogens?

back 17

they are multicellular organisms that are similar to humans

they are too big for phagocytosis

front 18

in general, what is the body's strategy to eliminate a parasite?

back 18

physical force for expulsion (coughing, sneezing, vomiting, diarrhea)

increased mucus for protection

front 19

what are the characteristics of type 2 immunity?

back 19

T(H)2 cells secrete cytokines that lead to IgE

parasite specific IgE antibody binds to FceR on basophils, eosinophils, and most cells

explosive reaction that dislodges that parasite from tissue that leads to expulsion

attempts to minimize collateral damage

front 20

example of type 2 immunity

back 20

IL-13 leads to mucous secretion; enterocyte turnover

front 21

early phase isotype switching

back 21

complement fixation

mast cell degranulation

phagocytosis

front 22

late phase isotype switching

back 22

immune complex formation

front 23

IgG4 preferentially binds to

back 23

FcyRIIB which is inhibitory

front 24

what are the unique structural/functional characteristics of IgE?

back 24

not soluble, but binds to FceRs

IgE has asymmetric shrimp shape that binds with high affinity

front 25

allergy associated with IgE

back 25

a type I hypersensitivity is an inappropriate IgE response against a harmless antigen

most individuals will have response against these antigens but it's usually another antibody isotype

front 26

two phases of hypersensitivity

back 26

the sensitization stage is the primary immune response to an antigen

the effector stage is a secondary immune response to the same antigen

front 27

what is omalizumab?

back 27

high affinity monoclonal antibody IgG specific for human IgE

recognizes and binds the Fc region of the IgE; binds soluble IgE but not bound IgE

prevents arming or sensitizing to the allergen

does not lead to susceptibility to disease

front 28

what are basophils?

back 28

basic-staining granulocytes: contain granules

similar developmental program to eosinophils, but are then reciprocally regulated

make up a very small percentage of leukocytes in the blood

front 29

how do basophils help initiate TH2 response?

back 29

secrete TH2 polarizing cytokines (IL-4 and IL-13)

express CD40L, which can bind to CD40 on B cells and drive isotype switching

front 30

what are eosinophils?

back 30

granules are loaded with arginine-rich basic proteins

named because they stain strongly with eosinophil

like mast cells, external stimulus causes them to release toxins and inflammatory mediators

reaction is highly toxic and damaging to host and parasite

front 31

eosinophilia

back 31

abnormally high numbers of eosinophils

damage to heart endocardium and to nerves

front 32

what are mast cells?

back 32

present in all vascularized tissues

maintain the integrity of the surrounding tissue

alert immune system to trauma and infection

repair damage caused by wounds and infection

recruit neutrophils, eosinophils, and effector T cells, and growth factors

front 33

what is the role of histamine in defense against parasites?

back 33

binds to one of four possible histamine receptors

acute allergic reactions are often caused by histamine binding to H1 of smooth muscle and endothelial cells

mast cells release TNFa which is complementary to histamine

histamine release is immediate

front 34

H1:

back 34

binding induces contraction of intestinal and bronchial smooth muscles, increased permeability of venules, and mucous secretion

front 35

H2

back 35

binding increases vasopermeability and vasodilation, stimulates exocrine glands, and increases stomach acid; also suppresses degranulation of mast cells/basophils in a negative feedback loop

front 36

H3

back 36

less involved in type 1: modulates neurotransmitter activity in CNS

front 37

H4

back 37

mediates mast cell chemotaxis

front 38

secondary mediators

back 38

formed when membrane phospholipids are enzymatically cleaved into eicosanoids

active at nanomle levels

the overall function of mast cells degranulation is to circulating leukocytes. to the site of activation to amplify the reaction initiated by antigen cross-linking of IgE

front 39

what is the purpose of tryptase, chymotryptase, and other proteases?

back 39

mast cells also make tryptase, chemotryptase, and other proteases; to help dislodge parasites

front 40

molecular type of inhaled allergens that favor type 2 immunity and IgE production

back 40

proteins; they induce T-cell responses

front 41

functions of inhaled allergens that favor type 2 immunity and IgE production

back 41

many allergens are proteases

front 42

low dose of inhaled allergens that favor type 2 immunity and IgE production

back 42

favors activation of IL-4 producing TFH2 cells

front 43

low molecular mass of inhaled allergens that favor type 2 immunity and IgE production

back 43

allows allergen to diffuse from particle to mucus

front 44

high solubility of inhaled allergens that favor type 2 immunity and IgE production

back 44

allergen readily elutes from particle

front 45

high stability of inhaled allergens that favor type 2 immunity and IgE production

back 45

allergen survives in desiccated particles

front 46

peptides presented by MHC class II of inhaled allergens that favor type 2 immunity and IgE production

back 46

needed for T-cell activation

front 47

what is sensitization?

back 47

the condition of a person who has been exposed once to an allergen and has made IgE antibodies against it

front 48

common examples of sensitization

back 48

dried feces of the dust mite

the specific antigen is a cysteine protease

circulated currents from HVAC

particles trapped in the mucous and carried to MHC II

front 49

predisposing environmental factors of allergy

back 49

air pollution

westernized countries

low gut microbial diversity

low fiber diet

front 50

protective environmental factors of allergy

back 50

farm environment

developing countries

high gut microbial diversity

high fiber diet

front 51

MHC class II alteration

back 51

affects coding sequence

enhanced presentation of allergen-derived peptides

front 52

T-cell receptor alpha chain alteration

back 52

noncoding

enhanced T-cell recognition of antigen-derived peptides

front 53

TIM family alteration

back 53

promoter and coding sequence

regulation of type 1/type 2 balance

front 54

IL-4 alteration

back 54

promoter altered IL-4 expression

front 55

IL-4 receptor alpha chain alterations

back 55

coding sequence

increased signaling in response to IL-4

front 56

high-affinity IgE receptor beta chain alteration

back 56

coding sequence

variation in the ligation of IgE by antigen

front 57

5-lipoxygenase alteration

back 57

promoter

variation in leukptriene production

front 58

what are important cytokines involved in allergy?

back 58

IL-4,3,9,12,13, GM-CSF

isotype switching, eosinophil survival, mast cell proliferation

front 59

what is atopy

back 59

the tendency to develop abnormal TH2 responses to harmless antigens

front 60

what are some examples of atopy?

back 60

allergic rhinitis

asthma

atopic dermatitis

food allergies

front 61

what is the wheel and flare?

back 61

a reaction observed when small amounts of allergen are injected into the dermis of an individual who is allergic to the antigen. it consists of a raised area of skin containing fluid (wheel) with a spreading, red, itchy reaction surrounding it (flare)

front 62

what is the effector phase?

back 62

the effects of IgE-mediated allergic reaction will vary according to the site of cell activation

front 63

early response

back 63

occurs within minutes of allergen exposure

mediated by mast cell granule release of histamine, leukptrienes, and prostaglandins

front 64

late response

back 64

occurs within hours later of allergen exposure

a result of recruited cells

cytokines released from mast cells increase expression of chemokines and CAMs on endothelium facilitating influx of neutrophils, eosinophils, and TH2

eosinophils play a large role in late-phase recruiting neutrophils and degranulation

front 65

what are the symptoms of allergies in the blood?

back 65

increased vascular permeability; constriction of smooth muscle; blood pressure reduction; swelling (edema) in connective tissues

leads to organ damage, impaired function, cause of death is often asphyxiation due to constriction of airway and swollen epiglottis

front 66

anaphylaxis

back 66

release of IgE leads to mass activation of mast cells and basophils via IgE -> FceR interactions

front 67

how is anaphylaxis treated?

back 67

stop exposure to the antigen

manage ABC

epinephrine injection

trendelenburg position

oxygen

antihistamines

corticosteroids

epinephrine

front 68

epinephrine

back 68

stimulates reformation of tight junctions in the vascular endothelium

reduces permeability

increased blood pressure

relaxes bronchial smooth muscle

stimulates the heart

front 69

allergic rhinitis

back 69

hay fever

allergens diffuse across mucous membrane and activate mucosal mast cells beneath it

local edema causing obstruction of the nasal airways and nasal discharge

front 70

allergic conjunctivitis

back 70

itchy, scratchy, watery eyes

similar to allergic rhinitis, but at the eye

front 71

allergic asthma

back 71

allergens activate submucosal mast cells in the lower airways of the respiratory tract

leads to mast-cell degranulation and increased secretion of fluid and mucus into the respiratory tract

bronchial constriction caused by contraction of smooth muscle

chronic inflammation

can lead to chronic asthma

front 72

what are examples of allergies in the skin?

back 72

atopic dermatitis (eczema)

atopic urticaria (hives)

front 73

how do food allergies occur?

back 73

ingestion of antigen activates mucosal mast cells

activated mast cells release histamine, which acts on epithelium, blood vessels, and smooth muscle

antigen diffuses into blood vessels and is widely disseminated, causing urticaria

front 74

how is type I sensitivity tested for?

back 74

skin testing is commonly used (it's cheap):

injection of small quantities of known allergens under skin

swelling and redness (resulting from local mast cell degranulation) indicate allergic response

front 75

how is type I treatments tested for?

back 75

antihistamines bind and block H1 receptors on target cells

leukotriene antagonists work in a manner similar to antihistamines

inhalation corticosteroids inhibit innate immune cell activity in airways

desensitization immunotherapy

front 76

blood transfusions (type II hypersensitivity)

back 76

blood group antigens are carbohydrates

people possess antibodies against the blood type they do not have

if an individual receives a transfusion of the "wrong" type of blood, their antibodies will quickly attach to the donor blood cells and trigger complement proteins

front 77

erythroblastosis fetalis (type II hypersensitivity)

back 77

hemolytic disease of the newborn; develops when maternal IgG antibodies specific to expressed RhD allele cross the placenta

destroys fetal RBCs by binding and activating complement; causes child to be anemic as well as a build-up of toxic RBC

front 78

hemolytic anemia (type II hypersensitivity)

back 78

can be drug induced

some drugs can absorb nonspecifically to proteins on RBC membranes

these drug-protein complexes may stimulate antibody production

antibodies then bind to RBCs when the drug is present, stimulating complement mediated destruction

penicillin can induce all four types of hypersensitivities under the correct circumstances for each other

front 79

what mediates destruction in type III hypersensitivity?

back 79

immune complexes

front 80

how does type III hypersensitivity occur?

back 80

if immune complexes aren't cleared effectively, they may deposit in tissues and can cause damage

may trigger release of inflammatory mediators and vasoactive mediators

proteases released may damage connective tissues

clots may form as complexes activate platelets

front 81

what are the symptoms of type III hypersenstivity?

back 81

fever, rashes, joint pain, lymph node, enlargement, and proteinuria

vasculitis if in blood vessel

autoantigens

glomerulonephritis if in kidney

arthritis in joints

front 82

what is contact dermatitis?

back 82

sensitization can occur if a reactive chemical compound binds to skin proteins, which are modified and then presented to T cells

could be induced by cosmetics, pharmaceuticals, industrial chemicals, metal ions, poison ivy, poison oak

can cause strong cell mediated responses against skin cells, inducing blister like lesions and rashes

front 83

what is chronic inflammation?

back 83

inflammation doesn't always resolve in a short period of time; it may last indefinitely, contributing to numerous other problems