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immunology lecture 18 key points

front 1

natural and active immunity

back 1

infection

front 2

artificial and active immunity

back 2

vaccine

front 3

natural and passive immunity

back 3

maternal antibodies

front 4

passive and artificial

back 4

antibody transfer

front 5

under what circumstances would you use passive immunization?

back 5

an individual with an immune deficiency

toxin/venom with an intermediate threat to life

individuals with immune-deficiencies

front 6

why wouldn't use you use passive immunization?

back 6

it doesn't activate the immune system; it's just a buffer

anti-isotypic antibodies could cause problems

if the need is prophylactic

front 7

vaccination

back 7

intentional exposure to an altered pathogen; also referred to as inoculation

front 8

immunization

back 8

the process of eliciting a state of protective immunity against a pathogen

front 9

vaccine valency

back 9

polyvalent vaccines are designed to confer protection against multiple strains of pathogen

front 10

contraindications

back 10

a specific state in which treatment, surgery, drug, vaccine shouldn't be used because it could be unsafe for a particular individual

front 11

herd immunity

back 11

when the majority of the population is immune to an infectious agent, the potential reservoirs for the pathogen are reduced. the chances of that pathogen coming contact with susceptible individual are greatly reduced

front 12

morbidity

back 12

state of illness

front 13

mortality

back 13

death

front 14

immunogenicity

back 14

the ability to provoke an immune response

front 15

efficacy

back 15

the therapeutic effectiveness of a vaccine

front 16

safety

back 16

the presence or absence of adverse events

front 17

adverse event

back 17

an unfavorable or unintended manifestation of disease that is associated with administration of the drug (vaccine)

front 18

variolation

back 18

an early method of inoculating a person against smallpox by intentionally exposing them to the virus, either by scratching pus from a smallpox sore into the skin or by blowing dried scabs into the nose

front 19

vaccination

back 19

injection of cowpox rather than smallpox

cross-reactivity

Louis Pasteur expanded the work to other infectious diseases

front 20

smallpox is the only disease to be completely eradicated, because:

back 20

it evolves slowly, antigens are conserved

the vaccine is a live virus that establishes infection at the site of injection; it mimics the innate and adaptive response

smallpox is human-specific; no reservoirs in other species

front 21

what are the three major goals of vaccination?

back 21

safety

efficacy

sustainability/achievability in target populations

front 22

in general, what correlates with immune protection?

back 22

basic research and rational design are important to advance vaccine development

vaccine development begins with basic research to discover immunogens

identification of specific immune targets or correlates of immune protection are crucial for full development

front 23

what is meant by rational vaccine design?

back 23

original strategies for vaccine production included: isolate, inactive, inject

rational design allows us to factor the genetic sequence and structure of the pathogen into the design of the vaccine

reverse vaccinology is the use of knowledge of a pathogen's physiology and how it exploits our immune system

front 24

live attenuated vaccine

back 24

the pathogen is alive, but is attenuated

grown under sub-optimal conditions; the pathogen survives and reproduces but can't cause harm

pathogenicity is lost, but immunogencitiy is retained

MMR, chickenpox, smallpox, rotavirus

front 25

what are the benefits of live attenuated vaccines?

back 25

the initial response is strong, large quantities of immunogen are made

they require relatively few booster immunizations

some are attenuated in certain species, but not in others

some can be produced by growing the pathogen in abnormal culture conditions

some can be genetically engineered

front 26

what are the drawbacks of live attenuated vaccines?

back 26

potential mutation back to virulent form

risk of some disease-related complications

requires cold-chain

recombinant DNA technology can now be used to make them safer

front 27

inactivated or killed vaccines

back 27

the whole pathogen is killed or inactived

the pathogen structure are preserved, but the pathogen cannot replicate or become virulent again

polio, hepatitis A, flu, rabies

front 28

what are the benefits of killed vaccines?

back 28

safer

more stable

they often don't require a cold-chain

front 29

what are the drawbacks of killed vaccines?

back 29

now as robust of a response

because they don't replicate, they require large quantities

because they're dead, they can't penetrate host cells, endogenous response is severely diminished

front 30

what is a toxoid vaccine?

back 30

the disease may be due to an exotoxin secretion and not due to the pathogen itself

toxoids are exotoxins that have been chemically altered such that lost their pathogenicity but retain their immunogenicity

examples include diphtheria and tetanus

front 31

what are the benefits toxoid vaccine?

back 31

neutralizing antibodies bind to the toxin and render it harmless

front 32

what are the drawbacks of toxoid immunity?

back 32

only works on those pathogens that secrete toxins

front 33

purified protein subunit vaccination

back 33

recombinant DNA technology can be used to make protein subunits

that protein can then be part of the vaccine formulation

these vaccines are often referred to as 'acellular'

examples include hepatitis B, pertussis

front 34

what are benefits purified protein subunit vaccination?

back 34

the recombinant DNA can be manipulated such that mutation can be introduced

front 35

what are the drawbacks of purified protein subunit vaccines?

back 35

the development process can alter the epitope such that immunogenicity or stability might be compromised - that might alter the type or number of neutralizing antibodies involved

like heat-killed vaccines, these cannot get into cells and therefore there is a limited cell-mediated response

front 36

purified carbohydrate subunit vaccines

back 36

made from purified polysaccharides of pathogens

examples include pneumococcus, meningococcus

front 37

what are the benefits of purified carbohydrate subunit vaccines?

back 37

easy to purify, because there are a lot of them

very few side effects

front 38

what are drawbacks of purified carbohydrate subunit vaccines?

back 38

carbohydrates do not generate T-dependent B cell responses often require a hapten conjugate

front 39

recombinant vector vaccines

back 39

genes for key antigens are inserted into attenuated virus

the virus acts a vector for the antigenic genes which are expressed by host expression machinery

examples include COVID19, HIV, RSV, Zika

front 40

what are the benefits of recombinant vector vaccines?

back 40

all the benefits of attenuated vaccines

fewer risks - not using the actual pathogen, little chance of reversion

the characteristics of the vector can be advantageous

front 41

what are the risks of recombinant vaccines?

back 41

immune responses against the vector

similar to attenuated viruses, particularly stability problems

front 42

vaccine excipient

back 42

an inactive molecule that serves as a support or aid to the drug but has no direct therapeutic impact

preservatives

adjuvants

stabilizers

cell culture materials

inactivating ingredients

antibiotics

front 43

what is an adjuvant?

back 43

molecules included to enhance the immune response to a vaccine, while limiting the amount of antigen administered

they can increase the inflammatory response

they can enhance antigen presentation

they can increase antigen stability

front 44

what are liposomes?

back 44

microscopic, spherical vesicles made of one or more phospholipid bilayers that can enclose an aqueous core

front 45

what is a booster vaccine?

back 45

booster vaccination is required to achieve protective immunity to many pathogens

front 46

what is VAERS?

back 46

vaccine adverse event reporting system

contact your healthcare provider

report an adverse event using the VAERS online form or the downloadable PDF

front 47

what are therapeutic vaccines?

back 47

treatment rather than prevention

chronic infection

allergy

chronic inflammation

front 48

coronavirus

back 48

type of virus

front 49

SARS-CoV-2

back 49

the specific virus

front 50

COVID19

back 50

the named disease cause by infection of the virus

front 51

how does coronavirus infect us?

back 51

an infected individual expels virus laden droplets which are then inhaled by a second

the inhaled virus finds its way to the upper respiratory tract where it utilizes a receptor known as angiotensin-converting enzymes 2 to enter host cellsS protein of virus attaches to ACE2

once inside it hijacks the cell's machinery

further infection results in movement down the respiratory tract

front 52

what is herd immunity

back 52

when enough people in an area have immunity to a disease that it no longer spreads easily. it usually takes a large number of people getting vaccinated against or infected with the germ to achieve herd immunity

front 53

how do mRNA vaccines work?

back 53

mRNA vaccines teach our cells how to make a protein that will trigger an immune response inside our bodies

like all vaccines, they benefit people who get vaccinated giving them protection against diseases without risking the potential serious consequences newly available to the public