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35 notecards = 9 pages (4 cards per page)

Viewing:

microbio lab final

front 1

What does it mean to turn the color blue in the oxidase test?

back 1

positive result (presence of cytochrome c oxidase in organism)

front 2

What is the name of a medium contains living microbes?

back 2

culture/growth medium

front 3

Which ID is this?

back 3

(idk lol)

front 4

What is the technique to isolate colonies with lower cell density samples?

back 4

streak plate method

front 5

What color are gram-positive cells on the Gram stain?

back 5

purple

front 6

Which drugs kill the bacteria?

back 6

antibiotics

front 7

What is the meaning of the I in ELISA test?

back 7

immunosorbent

ELISA (enzyme-linked immunosorbent assay)

front 8

What is this?

back 8

Close up egg with adult parasite

front 9

What changes in MacConkey agar to change the color of the agar?

back 9

fermentation of lactose (pink to red)

front 10

What do you identify in the oxidase test?

back 10

bacteria that possess the enzyme cytochrome c oxidase

front 11

What structure have the color seen in gram-negative cells?

back 11

thin peptidoglycan layer in their cell walls

front 12

What is the colloquial name for helminths?

back 12

parasitic worms

front 13

What do you use to sterilize the rod in the spread plate techniques?

back 13

alcohol and bunsen burner flame

front 14

What do you use to adjust the bacterial inoculum for turbidity?

back 14

McFarland standards

front 15

What type is Macconkey agar to lactose fermenters bacteria?

(WTF is this question)

back 15

- selective and differential media

- isolate and differentiate gram-negative bacteria based on their ability to ferment lactose

front 16

What is this?

back 16

helminth egg (this one is trichuris trichiura)

front 17

What is being measured?

back 17

zone of inhibition

front 18

Catalase Test

back 18

test used to determine if a bacteria produces the enzyme catalase

front 19

What preparation shows us the transparent bacteria?

back 19

Staining (simple stain, gram stain)

front 20

What is the name of the liquid we use to grow bacteria?

back 20

nutrient broth/culture medium

front 21

What segment of a worm, contains a complete sexually mature reproductive system.

back 21

proglottid

front 22

What do you use in hanging drop to form an airtight seal that slows the drying of the drop?

back 22

petroleum jelly

front 23

Some worms have separate sexes (male/female)

back 23

true (roundworms have seperate sexes while majority of other worms are hermaphrodites)

front 24

What is this?

back 24

proglottid of Taenia saginata

front 25

What is the technique to isolate colonies using 4 streaks with high cell density samples?

back 25

quadrant streak plate method

front 26

What should be done before using the loop to avoid contamination?

back 26

flame the loop

front 27

What is the name of the process by which competent bacterial cells collect DNA from the environment and use the genes it carries?

back 27

transformation

front 28

Structure that you saw in the sample

back 28

(microscope)

front 29

What is the meaning of the L in the ELISA test?

back 29

Linked

front 30

Order the steps to do the enterotube test

back 30

- remove the caps from both ends

- pick a colony with the inoculation wire

- inoculate all compartments

-break off the wire

-punch holes in the foil of the last eight compartments incubate

-interpret the results by comparing colors with the coding pad

front 31

When do you need to sterilize the rod in spread plate technique?

back 31

the rod or spreader needs to be sterilized before and after use and between streaking sections if multiple sections are used.

front 32

What do you see changing in the ELISA test if it is positive?

back 32

color change in the wells

front 33

What are the instances that allow a substance (germicide) to be registered and allowed on the market?

back 33

if it passes regulatory requirements set by agencies like the EPA and FDA, including submitting data on its efficacy, safety, and environmental impact

front 34

Which drug stops the bacteria from dividing but does not kill them?

back 34

bacteriostatic antibiotic

front 35

What is the meaning of the A in ELISA test?

back 35

Assay