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microbio lecture flashcards

front 1

What are the main features of Brightfield (Light) Microscopy?

back 1

• Most common type of light microscope

• Use white light to illuminate the sample from below.

• Widely used in biology and medical laboratories for examining
cell structures, tissues, and microorganisms.

Can view anywhere from 40x to 1000x magnification

Advantages: Simple, cost-effective, and suitable for observing
fixed and live specimens.

Limitations: Limited contrast for transparent or unstained
samples; not ideal for observing highly transparent specimens

front 2

What are the main features of Darkfield Microscopy?

back 2

• A variation of light microscopes that enhance contrast in unstained specimens (uses condenser)

• No staining is required making it ideal for observing live, unstained
specimens, such as bacteria, cells, and other small organisms.

• Use oblique light (light does not pass directly through the specimen)
but is instead scattered.

View range is 40x to 1000x magnification.

Advantages: Excellent for visualizing transparent or colorless
samples that are difficult to see with brightfield microscopy.

Limitations: Requires careful alignment; less effective with thick
specimens or samples that do not scatter light well.

front 3

What are the main features of Phase-Contrast Microscopy?

back 3

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