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36 notecards = 9 pages (4 cards per page)

Viewing:

microbio lab

front 1

Why don't you make any transfers over your books and paper?

back 1

You may inadvertently contaminate them

front 2

What is a medium that contains living microbes?

back 2

A culture

front 3

What do you need to do if you don't understand the instructions?

back 3

Ask the professor I am assuming

front 4

What do you use to obtain separated microcolonies?

back 4

Streak plate method

front 5

What can you see?

back 5

Gram-positive S.aureus

front 6

What kind of method is better when you have diluted samples?

back 6

Serial dilution method

front 7

What are the hanging drop steps in order?

back 7

-Place a small drop of bacterial culture on a coverslip,

-Seal the coverslip with petroleum jelly around the edge of a concave slide

-Then invert the slide with the droplet hanging in the depression, and observe under a microscope

front 8

How should you not handle glassware?

back 8

Carry glassware by its neck or side, use one hand to carry glassware, stand or walk on broken glass, put broken glass into a regular waste basket, etc.

front 9

In what type of bacteria can catalase be found?

back 9

Aerobic bacteria

front 10

What are the chemicals that develop color as they oxidize?

back 10

Tetramethyl-p-phenylenediamine (TMPD)

front 11

What kind of experiments can you do in the laboratory?

back 11

this is self explanatory

front 12

What can you see?

back 12

Gram-positive spirillum

front 13

What enzyme transforms hydrogen peroxide into water and gaseous oxygen?

back 13

Catalase

front 14

What kind of method uses 3 streaks?

back 14

T-streak method

front 15

What is tolerated in the laboratory?

back 15

Self explanatory

front 16

what can you see?

back 16

Gram-negative cocci

front 17

What is it called when you have a culture with two or more species?

back 17

Mixed culture

front 18

Which characteristic indicates that you prepare all the materials you need before starting the experiment and put it following the exercise procedure steps?

back 18

Being prepared?

front 19

What is a substance designed to reduce the number of pathogens on a surface or in liquids?

back 19

Chemical germicides

front 20

What is this? (in OF)

back 20

control

front 21

What is this? (in OF)

back 21

Fermentation, E.coli

front 22

What is this? (in OF)

back 22

control

front 23

In what type of bacteria can't catalase be found?

back 23

Obligate anaerobic bacteria

front 24

What method is this?

back 24

Zig-zag streak

front 25

In the oxidase reaction, what is the enzyme that changes the color?

back 25

Cytochrome oxidase

front 26

What is a substance designed to reduce the number of pathogens in living tissue?

back 26

Antiseptics

front 27

What method is this?

back 27

Quadrant streak

front 28

If you carry a microscope, what should you not do?

back 28

Do not grasp it by the stage, eyepieces, objective lenses, or other small parts.

Always hold it by the arm and base with both hands for proper support.

front 29

What kind of method uses 4 streaks?

back 29

Quadrant streak

front 30

What is this? (in MacConkey)

back 30

Non-lactose fermenting colonies

front 31

What is the shape of your stain sample?

back 31

How to do a simple stain:

1. Sterilize inoculating loop

2. Obtain your sample and spread it on the slide

3. Allow the sample to air dry for 1 min

4. Fix the sample to the slide (pass over the flame)

5. Flood sample with simple stain (crystal violet, safranin, methylene blue)

6. Let the stain sit for 1 min

7. Rinse with DDH2O

8. Place the coverslip onto the slide

9. Look under the microscope

front 32

What can you see?

back 32

Gram-negative rods

front 33

What word do we use for something without contamination?

back 33

Uncontaminated

front 34

What is a substance designed to reduce the number of pathogens on a surface?

back 34

Disinfectants

front 35

What can you see?

back 35

Gram-positive rods

front 36

What can you do in the laboratory?

back 36

Self-explanatory