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EXCERCISE 5.5 LAB MICROBIO

front 1

1.What type of inclusions are easy to spot by negative staining?
A) Protein inclusions
B) Carbohydrate inclusions
C) Refractile cytoplasmic inclusions
D) Nucleic acid inclusions

back 1

C) Refractile cytoplasmic inclusions

front 2

1.What is the main advantage of using negative staining?
A) It stains the cell wall
B) It is a quick procedure
C) It requires heating the specimen
D) It uses basic dyes

back 2

B) It is a quick procedure

front 3

1.What can negative staining be used to demonstrate?
A) Intracellular structures
B) Extracellular structures
C) Genetic material
D) Cell division

back 3

B) Extracellular structures

front 4

1.What type of layer do many bacteria secrete outside of their cell wall?
A) Protein layer
B) Lipid layer
C) Mucoid layer of polysaccharide or polypeptide
D) Nucleic acid layer

back 4

C) Mucoid layer of polysaccharide or polypeptide

front 5

1.How do encapsulated bacteria usually appear in colony morphology?
A) Dry and rough
B) Gooey, slimy, smooth, and shiny
C) Flat and dull
D) Irregular and fuzzy

back 5

B) Gooey, slimy, smooth, and shiny

front 6

1.How can the presence of capsules be confirmed?
A) By using Gram staining
B) By using capsule stains or negative staining
C) By using simple staining
D) By using endospore staining

back 6

B) By using capsule stains or negative staining

front 7

1.What appearance does the capsule have when smears of encapsulated cells are prepared with Congo red?
A) Colored halo surrounding the cell
B) Clear halo surrounding the cell
C) Dark spot within the cell
D) No visible change

back 7

B) Clear halo surrounding the cell

front 8

1.Why are some bacteria difficult to see without special staining techniques?
A) They are too large
B) They have a thick cell wall
C) They secrete extracellular polymers
D) They are highly motile

back 8

C) They secrete extracellular polymers