front 1 1.What is the primary use of the negative stain?
A) To
determine the chemical composition of a specimen
B) To determine
the shape and arrangement of a specimen
C) To identify the
genetic material of a specimen
D) To measure the size of a specimen | back 1 B) To determine the shape and arrangement of a specimen |
front 2 1.Why is there less distortion of the size and arrangement of the
organism in negative staining?
A) Because the specimen is heated
B) Because the specimen is not heated
C) Because the
specimen is chemically treated
D) Because the specimen is frozen | back 2 B) Because the specimen is not heated |
front 3 1.For which type of organisms is negative staining particularly
useful?
A) Organisms that are easily stained with positive
stains
B) Organisms that are not easily stained with positive
stains
C) Organisms that are heat-resistant
D) Organisms
that are chemically inert | back 3 B) Organisms that are not easily stained with positive stains |
front 4 1.What principle is negative staining based on?
A) A dye with a
positive charge is attracted to the bacterial cell
B) A dye with
a negative charge is repelled by the bacterial cell
C) A dye
with a neutral charge is absorbed by the bacterial cell
D) A dye
with a negative charge is absorbed by the bacterial cell | back 4 B) A dye with a negative charge is repelled by the bacterial cell |
front 5 1.Why do some organisms repel the stain in negative staining?
A) Because of the high protein content in their cell walls
B) Because of the chemical nature of their cell walls
C)
Because of the high carbohydrate content in their cell walls
D)
Because of the high nucleic acid content in their cell walls | back 5 B) Because of the chemical nature of their cell walls |
front 6 1.Which dye is commonly used in the negative staining procedure?
A) Crystal violet
B) Safranin
C) Nigrosin
D)
Methylene blue | |
front 7 1.What is the appearance of the background and the cell after
negative staining?
A) The background is colorless, and the cell
is black
B) The background is black, and the cell is colorless
C) Both the background and the cell are black
D) Both the
background and the cell are colorless | back 7 B) The background is black, and the cell is colorless |
front 8 1.What is the charge of the dye used in negative staining?
A)
Positive
B) Negative
C) Neutral
D) Variable | |
front 9 1.Which component of Mycobacterium's cell wall contributes to its
ability to repel the stain?
A) Proteins
B) Carbohydrates
C) Lipids
D) Nucleic acids | |
front 10 1.What is the main advantage of using negative staining for delicate
organisms?
A) It enhances the color of the organisms
B) It
prevents distortion of the organisms
C) It increases the size of
the organisms
D) It chemically alters the organisms | back 10 B) It prevents distortion of the organisms |