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EXCERCISE 5.3 LAB MICROBIO

front 1 1.What should be done if Gram-positive organisms are staining as Gram-negative? A) Increase the decolorization time B) Lengthen the crystal violet and mordant step and shorten the decolorizer step C) Use a different stain D) Use older cultures	back 1 B) Lengthen the crystal violet and mordant step and shorten the decolorizer step
front 2 1.What is a possible cause if no organism is visible? A) Overheating the smear B) Failure to heat fix C) Using too many organisms D) Using old culture	back 2 B) Failure to heat fix
front 3 1.What could cause organisms to appear clumped on a slide? A) Too many organisms on slide B) Failure to heat fix C) Over decolorized D) Improper decolorization	back 3 A) Too many organisms on slide
front 4 1.If the smear appears granular under oil, what might be the cause? A) Overheating the smear B) Using old culture C) Lens is dirty or condenser lens too low D) Excessive rubbing of smear	back 4 C) Lens is dirty or condenser lens too low

front 5 1.What should be done if Gram-positive organisms appear pink? A) Use fresh culture B) Increase time with crystal violet C) Decolorize longer D) Apply heat when doing primary stain	back 5 B) Increase time with crystal violet
front 6 1.What could cause Gram-negative organisms to appear blue? A) Over decolorized B) Improper decolorization C) Using old culture D) Failure to heat fix	back 6 B) Improper decolorization
front 7 1.What should be done if no spores are visible after a spore stain? A) Use older culture B) Use fresh reagents C) Increase time with crystal violet D) Decolorize longer	back 7 B) Use fresh reagents
front 8 1.What might cause Gram-positive organisms to appear too faint? A) Crystal violet stain time is too short B) Over decolorized C) Improper decolorization D) Failure to heat fix	back 8 A) Crystal violet stain time is too short

front 9 1.Why is the acid-fast stain named as such? A) Because it uses acidic dyes B) Because it resists decolorization by acids C) Because it stains acidic bacteria D) Because it uses acid to fix the smear	back 9 B) Because it resists decolorization by acids
front 10 1.What component in Mycobacteria cell walls contributes to their acid-fastness? A) Proteins B) Carbohydrates C) Lipids and mycolic acids D) Nucleic acids	back 10 C) Lipids and mycolic acids
front 11 1.In the Ziehl–Neelsen method, what is the purpose of placing the slide on a staining rack containing boiling water? A) To fix the smear B) To heat the carbolfuchsin C) To decolorize the smear D) To dry the smear	back 11 B) To heat the carbolfuchsin
front 12 1.How long should the smear be covered with carbolfuchsin in the Ziehl–Neelsen method? A) 1-2 minutes B) 3-4 minutes C) 5-7 minutes D) 8-10 minutes	back 12 C) 5-7 minutes

front 13 1.What is used to decolorize the smear in both the Ziehl–Neelsen and Kinyon methods? A) Ethanol B) Acid alcohol C) Acetone D) Water	back 13 B) Acid alcohol
front 14 1.What is the counterstain used in both the Ziehl–Neelsen and Kinyon methods? A) Crystal violet B) Safranin C) Methylene blue D) Malachite green	back 14 C) Methylene blue
front 15 1.In the Kinyon method, how long should the smear be decolorized initially? A) 1 minute B) 2 minutes C) 3 minutes D) 4 minutes	back 15 B) 2 minutes
front 16 1.What is the appearance of acid-fast bacilli after staining? A) Blue with a red background B) Red with a blue background C) Green with a blue background D) Purple with a pink background	back 16 B) Red with a blue background

front 17 1.What would be the Gram stain reaction of Mycobacterium? A) Gram-positive B) Gram-negative C) Acid-fast D) Non-reactive	back 17 C) Acid-fast
front 18 1.How would organisms other than Mycobacterium appear when subjected to acid-fast staining? A) Red B) Blue C) Green D) Purple	back 18 B) Blue