front 1 1.What is the primary instrument for viewing bacteria in a
microbiology lab? | back 1 B) Compound microscope |
front 2 1.How is total magnification calculated in a compound microscope?
| back 2 B) Objective x ocular |
front 3 1.What happens to the working distance as the magnifying power of the
lens increases? | back 3 B) It decreases |
front 4 1.What does RP (resolution) refer to in microscopy? | back 4 B) Ability to distinguish two nearby points as separate |
front 5 1.What is the numerical aperture (NA) of a lens? | back 5 B) The light-gathering capacity of the lens |
front 6 1.How should a microscope be carried? | back 6 B) With both hands, one holding the arm and one under the base |
front 7 1.What should be used to clean the oculars, objectives, and condenser
lenses? | back 7 D) Lens cleaner |
front 8 1.What should be done at the end of each microscope session? | back 8 B) Remove the slide, wipe away the oil, and place the scanning objective in a vertical position |
front 9 1.What is the purpose of staining in microbiology? | back 9 B) To increase contrast between bacteria and the slide |
front 10 1.What is the first step in smear preparation from a plate or broth?
| back 10 B) Begin with a clean, dry slide |
front 11 1.What is the purpose of heat fixing a slide? | back 11 B) To kill the bacteria and make them adhere to the slide |
front 12 1.What stain is used in the simple stain procedure? | back 12 B) Methylene blue or Safranin |
front 13 1.What is the purpose of the negative stain procedure? | back 13 B) To stain the background and leave the bacteria unstained |
front 14 1.What is used to spread the drop of bacteria and stain in the
negative stain procedure? | back 14 B) The edge of a second clean slide |
front 15 1.What is the purpose of a wet mount? | back 15 B) To observe living organisms in a liquid environment |
front 16 1.What should be used to cover the drop of hay infusion liquid in a
wet mount? | back 16 C) A coverslip |