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Microbiology Final Lab Exam

front 1

What is the purpose of heat-fixing the smear?

back 1

to kill the organisms/bacteria and to attach them to the slide

front 2

Which part of the specimen (cell or background) is stained in a Simple Stain?

back 2

the cell

front 3

Would you have gotten the same result if you used an anionic (-charged) stain?

back 3

No

front 4

Do you obtain an accurate estimate of cell size with a simple stain?

back 4

No

front 5

For which step is the timing most critical?

back 5

the decolorizing step because over decolorization may occur

front 6

What would you expect to observe if the Simple Stain procedure rather than the Gram stain had been used on a smear containing a mixture of gram-positive cocci and gram negative rods?

back 6

the cells would all be the same color

front 7

Microorganisms are best defined as organisms that

back 7

are too small to be seen with the unaided eye

front 8

Among the types of microorganisms, the ______ are noncellular.

back 8

Viruses

front 9

Unable to obtain sharp focus using oil immersion objective lens. What is the possible cause?

back 9

Condenser is too low

front 10

If a microbiology lab technician left the safranin out of the Gram stain procedure, what would be the result?

back 10

the Gram-positive cells would remain purple but the Gram-negative cells would be colorless

front 11

A pure culture contains

back 11

only one identified species of microorganism

front 12

What is the term for a culture made from one isolated colony?

back 12

Both axenic and pure