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CBNS101 Lecture 3: Simple Membrane Transport Channels, Transporters, Synaptic Transmission

front 1

Membrane transport proteins make up _____% of all membrane proteins in the cell

back 1

15-30%

front 2

List some small nonpolar molecules

back 2

O2 and CO2

front 3

List some small uncharged polar molecules

back 3

H2O, glycerol and ethanol

front 4

What are some molecules that cannot penetrate the cell membrane by diffusion?

back 4

Large uncharged polar molecules, ions and any charged molecules

front 5

How do ions and small polar molecules get past the cell membrane?

back 5

Through transport proteins that are specific and choosy of what goes through them

front 6

What disease causes an inability to transport amino acids like cysteine from urine or intestine into the blood/ Inevitably contributing to kidney stones.

back 6

Cystinuria disease

front 7

A narrow hydrophilic pore that allows diffusion of specific ions along an electrochemical gradient

back 7

Channel proteins

front 8

Proteins that bind to the molecule and undergo a conformational change that exposes binding sites for the molecule on different sides of the membrane.

back 8

Transporter

front 9

T/F An electrochemical gradient is the potential difference in both concentration and electric potential

back 9

True

front 10

T/F Only channels mediate passive transport

back 10

False. Both transporters and channels mediate passive transport

front 11

What is Antiport?

back 11

The co-transport of ions in opposite direction

front 12

What is a symport?

back 12

The co-transport of ions in the same direction

front 13

What is a uniport?

back 13

The transport of a single solute (not coupled)

front 14

These pumps are single sub-unit proteins. They phosphorylate themselves and pump ions.

a) P-Type pumps

b) ABC transporters

c) V-Type pumps

d) F-Type pumps

back 14

a) P-Type pumps

front 15

These pumps are made up of multiple subunits. They pump many different small molecules.

a) P-Type pumps

b) ABC transporters

c) V-type pumps

d) F-Type pumps

back 15

b) ABC Transporters (ATP-binding-cassette)

front 16

These pumps are made up of multiple subunits. They pump H+ into organelles.

a) P-Type pumps

b) ABC transporters

c) V-type pumps

d) F-Type pumps

back 16

V-Type pumps (like in vacuoles)

front 17

These pumps use the H+ gradient to synthesize ATP

a) P-Type pumps

b) ABC transporters

c) V-type pumps

d) F-Type pumps

back 17

F-Type pumps (work in the reverse of V-Type)

front 18

Review Slides

back 18

11, 12, 14,

front 19

What are the names of the water channels that transport water? Water will quickly and passively move through these types of channels

back 19

Aquaporins

front 20

_____ channels conduct way better

back 20

K+

front 21

The Na+-K+ pump produces gradients of high Na+ ______ and high K+ ________ and set up a charge imbalance

back 21

Na+ outside and K+ inside

front 22

Describe the process of establishing a membrane potential

back 22

The Na+-K+ pump pumps Na+ outside the cell and K+ inside the cell. There is a K+ leak channel for K+ to leak outside, making the inside of the cell slightly more negative. Once the (+) and (-) turn the inside neutral that is called the resting membrane potential

front 23

When the membrane is polarized (at resting membrane potential), these channels are closed. When the inside is depolarized (less negative inside), they open. What ion channel is this?

back 23

Voltage-gated ion channel

front 24

This channel is stimulated from mechanical movement or stress

back 24

Mechanically gated ion channel

front 25

This channel is activated when bound to another molecule. It can be both intracellular or extracellular.

back 25

Ligand-gated ion channels

front 26

These neurotransmitters open nonselective cation channels allowing an influx of Na+ to depolarize the membrane. Usually taking multiple of these signals to do so.

back 26

Excitatory neurotransmitters

front 27

These neurotransmitters open the Cl- channels to hyperpolarize the membrane (harder to make the neuron fire)

back 27

Inhibitory neurotransmitters

front 28

Just know this fact I guess

back 28

the stronger the signal, the faster the firing