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  3. Cut out the notecards by cutting along each horizontal and vertical dotted line
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16 notecards = 4 pages (4 cards per page)

Viewing:

genetics quiz 4??

front 1

southern blotting

back 1

synthesizes DNA

front 2

northern blotting

back 2

synthesizes RNA

front 3

western blotting

back 3

synthesizes protiens

front 4

1st step PCR

back 4

Primers target a specific region

front 5

2nd step PCR

back 5

Denature DNA (~95°C)

front 6

3rd step PCR

back 6

Anneal primers (~55°-65°C)

front 7

1st step cDNA synthesis

back 7

RNA purification

front 8

2nd step cDNA synthesis

back 8

RNA incubated with reverse transcriptase

front 9

3rd step of cDNA synthesis

back 9

oligo-dT primer anneals polyA tail of mRNA so reverse transcriptase synthesizes single strands DNA

front 10

4th step of cDNA synthesis

back 10

mRNA is removed and DNA polymerse copies the cDNA strand

front 11

5th step of cDNA synthesis

back 11

nick hairpin

front 12

4th step PCR

back 12

Elongate new DNA strands, using Taq or other DNA polymerase(~72°C)

front 13

5th step PCR

back 13

Repeat denaturation, annealing, and elongation for 20-40 cycles.

front 14

miRNA and siRNA similarities

back 14

21-22 nucleotides

undergo post-transcriptional processing.
involve the formation of double-stranded RNA

participate in RNA interference (RNAi), targeted, RNA-
mediated repression of gene expression

front 15

siRNAs

back 15

no data

front 16

miRNA

back 16

Many are produced by RNA pol II

regulate other genes

may have a mismatch with target transcripts

are implicated in developmental processes

may block translation and/or trigger RNA degradation