front 1 What differentiates a eukaryotic mRNA strand from its precursor
RNA? | back 1 A. Introns are spliced out |
front 2 T/F: 5’ to 3’ of mRNA corresponds to N-to-C terminus in
polypeptides | back 2 A. True |
front 3 List other names for the non-template strand of DNA. | back 3 Coding strand, RNA-like strand, sense strand |
front 4 Which two are the correct pairings of promotes sequences and their
locations? | back 4 B. TATAAT (Pribnow box), -10 bp |
front 5 If the template strand of DNA is 5’ATTGGTAGCT3’, what is the sense strand? What is the resulting mRNA strand? | back 5 Sense strand: 5’AGCTACCAAT3’ |
front 6 Which of the following is NOT true about sigma factors? | back 6 A. Eukaryotic RNA Polymerase recognizes it |
front 7 T/F: Promotors can vary in strength | back 7 A. True |
front 8 Which of the following is NOT true regarding initiation of
prokaryotic transcription? | back 8 C. Sigma factor remains bound to the RNA polymerase complex throughout the transcription process |
front 9 T/F: Only one RNA polymerase can bind to a particular DNA strand at a
time. | back 9 B. False |
front 10 Describe Rho-dependent termination of prokaryotic transcription. | back 10 - Rho factor binds to the rut site on RNA transcript and moves
towards 3’ end of the transcript |
front 11 T/F: Both Rho independent and dependent termination involve reaching
a | back 11 A. True |
front 12 Which of the following is true regarding Rho-independent termination
of prokaryotic | back 12 D. All of the above are true |
front 13 T/F: Eukaryotic mRNA is usually polycistronic in nature | back 13 B. False |
front 14 Which of the following is FALSE regarding eukaryotic
transcription? | back 14 B. The core promoter is located at the -10 region |
front 15 Which of the following is true regarding eukaryotic
transcription? | back 15 D. All of the above are true |
front 16 Describe how RNA Polymerases I-III terminate transcription in Eukaryotes: | back 16 I: requires a termination factor similar to rho. Binds to DNA here NOT RNA!! ll:Termination of transcription has no specific
sequences. III: ends transcription after terminator sequence w/multiple Us |
front 17 Which FOUR statements below are correct? E. In Eukaryotes, RNA splicing occurs after the mRNA is transported
into the | back 17 C. In prokaryotes, transcription is coupled to translation F. RNA splicing requires the formation of a spliceosome |
front 18 T/F: In eukaryotes, one pre-mRNA strand can only result in one
protein. | back 18 B. False - alternative splicing! |
front 19 T/F: The promotor region is located downstream of the transcription
start site? | back 19 B. False - upstream! |
front 20 T/F: mRNA is transcribed from the sense strand of DNA, making the
two | back 20 B. False - antisense! |
front 21 Carefully read the following double stranded DNA. Locate the
consensus sequence in 21. What is this consensus sequence called? | back 21 Pribnow Box |
front 22 Carefully read the following double stranded DNA. Locate the
consensus sequence in 22. Is this DNA prokaryotic or eukaryotic? | back 22 Prokaryotic |
front 23 Carefully read the following double stranded DNA. Locate the
consensus sequence in 23. Where is it located relative to the start of transcription? | back 23 10 Nucleotides upstream |
front 24 24. What is this sequence’s equivalent in either prokaryotes or eukaryotes? | back 24 TATA box |
front 25 25. Which of the following causes DNA and RNA to separate during Rho
dependent | back 25 B. Rho’s helicase activity |