front 1 Mark all that have a prokaryotic cell type | back 1
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front 2 Mark all that have a eukaryotic cell type | back 2
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front 3 Mark all that are animals | back 3
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front 4 Which gene is used to molecularly identify bacteria and archaea? | back 4 16s rRNA |
front 5 Which gene is used to molecularly identify Eukarya? | back 5 ITS |
front 6 Which objective lens should be in position when placing a slide onto the stage or off the stage? | back 6 4x |
front 7 Total magnification power is calculated by multiplying the objective power in use by | back 7 the ocular power (10x) |
front 8 Which part of the microscope can be changed to make a brightfield microscope a darkfield microscope? | back 8 Condenser |
front 9 Which part of the microscope can be changed to make a brightfield microscope a phase contrast microscope? | back 9 Diaphragm |
front 10 When converting the field of view from mm to μm, the mm measurement is multiplied by which value? | back 10 1000 |
front 11 The simple staining method that uses one of the acidic dyes such as nigrosin or India ink | back 11 Negative Staining |
front 12 The simple staining method that uses a single acidic or basic stain | back 12 Positive staining |
front 13 The differential staining method that uses crystal violet and safranin | back 13 Gram staining |
front 14 The differential staining method that uses fuchsin and methylene blue | back 14 Acid-fast staining |
front 15 The differential staining method that uses malachite green and safranin | back 15 Spore staining |
front 16 With negative staining, what color will bacterial colonies appear as? | back 16 White |
front 17 With spore staining, endospores will appear as what color? | back 17 Green |
front 18 With acid-fast staining, Mycobacterium (acid-fast bacteria) will stain which color because of the presence of mycolic acid? | back 18 Red |
front 19 With Gram staining, Gram positive bacteria will stain as which color? | back 19 Purple/blue |
front 20 With Gram staining, Gram negative bacteria will stain as which color? | back 20 Pink |
front 21 Agar, a molecule derived from red algae, is an important ingredient in which type of media? | back 21 Semi-solid |
front 22 This type of media contains one or more agents that inhibit the growth of certain microbes but not another | back 22 Selective |
front 23 These media are designed to grow a broad spectrum of microbes that do not have special growth requirements | back 23 General purpose |
front 24 This type of media can grow several types of microbes but are designed to bring out visible differences among those microbes | back 24 Differential |
front 25 This media is a general purpose media used for nonfastidious microbes including many bacteria and fungi | back 25 Nutrient Agar or Broth (NA & NB) |
front 26 This selective and differential media is used to isolate enteric bacteria that hydrolyze urea | back 26 Eosin Methylene Blue Agar (EMB) |
front 27 This media is a general purpose media used for bacteria | back 27 Trypticase Soy Agar (TSA) |
front 28 This media is a general purpose media used for fungi | back 28 Potato Dextrose Agar (PDA) |
front 29 This selective and differential media is used to isolate enteric bacteria that ferment lactose | back 29 MacConkey's Agar |
front 30 This selective and differential media is used to isolate Staphylococcus | back 30 Mannitol Salt Agar (MSA) |
front 31 MSA is a selective medium for which bacteria because of the high salt concentration? | back 31 Staphylococcus sp. |
front 32 MSA will turn what color when the bacterium ferments the mannitol and produces acid? | back 32 Yellow |
front 33 EMB agar is selective for which bacteria? | back 33 Gram-negative intestinal pathogenes |
front 34 EMB agar is selective because only bacteria that ferment this sugar produce colored colonies | back 34 Lactose |
front 35 Colonies of E. coli on EMB agar will produce what color colonies? | back 35 Green |
front 36 Colonies of Enterobacter aerogenes on EMB agar will produce what color colonies? | back 36 Pink |
front 37 MacConkey's agar is selective for what group of bacteria? | back 37 Salmonella sp. |
front 38 Colonies of coliform bacteria on MacConkey's agar will produce what color colonies? | back 38 Red |
front 39 The ingredients of crystal violet and bile salts inhibit which group of bacteria? | back 39 Gram-positive intestinal pathogenes |
front 40 If the nutrient agar is neither selective or differential, then the colonies of the different bacteria should look | back 40 Similar |
front 41 Cell division for bacteria is known as | back 41 Binary fission |
front 42 In this phase, the cells are adjusting to their environment | back 42 Lag phase |
front 43 In this phase, the cells reach the maximum rate of cell division | back 43 Log phase |
front 44 In this phase, the cells will stop growing or grow very slowly | back 44 Stationary phase |
front 45 In this phase, limiting factors intensify becoming detrimental to any cell growth | back 45 Death phase |
front 46 Generation time should be measured during which phase? | back 46 Log phase |
front 47 A dilution of 1:10,000,000 is equivalent to 10 to which exponent? | back 47 7 |
front 48 A dilution of 1:1,000,000 is equivalent to 10 to which exponent? | back 48 6 |
front 49 When plating out a serial dilution series, which solution would have the fewest colony forming units? | back 49 1:10,000,000 |
front 50 An increase in optical density or absorbance indicates _______ microbial organisms | back 50 an increase in the number of |
front 51 Bacteria that must grow in oxygen because their metabolism requires oxygen | back 51 Obligate aerobes |
front 52 Bacteria that have a flexible metabolism for oxygen conditions | back 52 Facultative anaerobes |
front 53 Bacteria that cannot grow in an oxygenated environment | back 53 Obligate anaerobes |
front 54 Many bacteria pathogens have an optimal growth between 35°C and 40°C, these are classified as | back 54 Mesophiles |
front 55 Which bacteria's colonies change color from white (higher temperature) to red (lower temperature)? | back 55 Serratia |
front 56 pH refers to what? | back 56 Concentration of hydrogen ions |
front 57 A cell that has fewer solutes than its environment | back 57 Hypotonic |
front 58 A cell that has the same solute concentration as its environment | back 58 Isotonic |
front 59 A cell that has more solutes than its environment | back 59 Hypertonic |
front 60 A microbe such as Halobacterium salinarium thrives in high salt concentration environments is known as | back 60 Obligate halophile |