front 1 What is principle behind dark field microscopy? | back 1 Opposite of light microscopy undiffracted light doesn't pass through specimen resulting in bright image against dark background useful in viewing UNSTAINED organisms |
front 2 When would you use dark field microscopy | back 2 Wen observing unstained living microorganisms or microorganisms that are hard to stain and spirochetes that are poorly defined in bright field |
front 3 Bright field | back 3 Uses light from lamp under stage to focus in plane of specimen |
front 4 Dark field | back 4 Condenser forms hollow cone of light objective lense sits in dark hollow cone and light travels around objective lense like backlighting |
front 5 What is function of abbé condenser in dark field microscopy | back 5 Directs light away makes dark |
front 6 Function of dark field stop | back 6 To turn the compound microscope into a dark field microscope |
front 7 How is compound mic turned into dark field mic | back 7 Fitting it with dark field condenser that has a resolving power greater than the objective
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