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7 notecards = 2 pages (4 cards per page)

Viewing:

Dark field light microscope lab 3

front 1

What is principle behind dark field microscopy?

back 1

Opposite of light microscopy undiffracted light doesn't pass through specimen resulting in bright image against dark background useful in viewing UNSTAINED organisms

front 2

When would you use dark field microscopy

back 2

Wen observing unstained living microorganisms or microorganisms that are hard to stain and spirochetes that are poorly defined in bright field

front 3

Bright field

back 3

Uses light from lamp under stage to focus in plane of specimen

front 4

Dark field

back 4

Condenser forms hollow cone of light objective lense sits in dark hollow cone and light travels around objective lense like backlighting

front 5

What is function of abbé condenser in dark field microscopy

back 5

Directs light away makes dark

front 6

Function of dark field stop

back 6

To turn the compound microscope into a dark field microscope

front 7

How is compound mic turned into dark field mic

back 7

Fitting it with dark field condenser that has a resolving power greater than the objective
Light now passing through the specimen is diffracted and enters objective lense whereas undiffracted light doesn't results in bright image against dark background