40 notecards = 10 pages (4 cards per page)
Assume you inoculated 100 facultatively anaerobic cells onto nutrient agar and incubated the plate aerobically. You then inoculated 100 cells of the same species onto nutrient agar and incubated the second plate anaerobically. After incubation for 24 hours, you should have
the same number of colonies on both plates.
The term trace elements refers to
small mineral requirements.
Which one of the following temperatures would most likely kill a mesophile?
Which of the following is not a characteristic of biofilms?
Which of the following types of media would not be used to culture aerobes?
An organism that has peroxidase and superoxide dismutase but lacks catalase is most likely an
Which of the lines best depicts the log phase of Listeria monocytogenes growing in a human?
Which of the lines best depicts the log phase of a thermophile incubated at room temperature?
Medium 2 is
Medium 1 is
selective and differential
Which of the following does not kill endospores?
Which of the following is most effective for sterilizing mattresses and plastic Petri dishes?
Which of these disinfectants does not act by disrupting the plasma membrane?
Which of the following cannot be used to sterilize a heat-labile solution stored in a plastic container?
Which of the following is used to control microbial growth in foods?
Which disinfectant(s) is (are) bactericidal?
A, C, and D
Which of the following is not a characteristic of quaternary ammonium compounds?
A classmate is trying to determine how a disinfectant might kill cells. You observed that when he spilled the disinfectant in your reduced litmus milk, the litmus turned blue again. You suggest to your classmate that
Which of the following is most likely to be bactericidal
Transfer of DNA from a donor to a recipient cell by a bacteriophage.
Transfer of DNA from a donor to a recipient as naked DNA in solution.
Feedback inhibition differs from repression because feedback inhibition
stops the action of preexisting enzymes.
Bacteria can acquire antibiotic resistance by all of the following except
Suppose you inoculate three flasks of minimal salts broth with E. coli. Flask A contains glucose. Flask B contains glucose and lactose. Flask C contains lactose. After a few hours of incubation, you test the flasks for the presence of β-galactosidase. Which flask(s) do you predict will have this enzyme?
Plasmids differ from transposons in that plasmids
are self-replicated outside the chromosome.
Mechanism by which the presence of glucose inhibits the lac operon.
The mechanism by which lactose controls the lac operon.
Two offspring cells are most likely to inherit which one of the following from the parent cell?
a change in a nucleotide in DNA
Which of the following is not a method of horizontal gene transfer?
Restriction enzymes were first discovered with the observation that
phage DNA is destroyed in a host cell.
Which of the following is the fourth basic step to genetically modify a cell?
The following enzymes are used to make cDNA. What is the second enzyme used to make cDNA?
if you put a gene in a virus, the next step in genetic modification would be
You have a small gene that you want replicated by PCR. You add radioactively labeled nucleotides to the PCR thermal cycler. After three replication cycles, what percentage of the DNA single strands are radioactively labeled?
Pieces of human DNA stored in yeast cells.
A population of cells carrying a desired plasmid.
Self-replicating DNA for transmitting a gene from one organism to another.
DNA that hybridizes with mRNA.