Use of Ninhydrin Reaction

Helpfulness: 0
Set Details Share
created 3 years ago by chemistsquirrel
12 views
Ninhydrin reagents, identifying amino acids
show moreless
Page to share:
Embed this setcancel
COPY
code changes based on your size selection
Size:
X
Show:
1

Ninhydrin reagent is used for qualitative and quantitative analysis of ( ).

amino acids

2

using polar silica gel as a stationary phase, which of the following runs faster?

Leucine

3

Rf is a constant characteristic of a solute.

true

4

which of the following materials will not be used in the experiment?

NaOH.

5

Rf value can be changed with which of the following?

solvent, stationary phase, temperature

6

opponents of aspartame claimed that it is a health hazard because which of the following substance hydrolyzed by aspartame?

methanol

7

The nonpolar molecules which have high affinity with the polar silica gel (a stationary phase) are held tightly and therefore run faster than polar molecules on TLC plates.

false. it's polar molecules which have a high affinity for silica gel.

8

The dehydrated form of Ninhydrin forms a Schiff's base with _______ groups of amino acids, peptides, and proteins. Under ________ and ___________ conditions, decarboxylation of the amino acid portion of the Schiff base occurs. This is followed by dissociation of an aldehyde, which generates a primary amine that reacts with yet another molecule of the reduced form of Ninhydrin to produce a/an _________ color product.

  • a-amino
  • hot and weakly acidic
  • purple
9

TLC employs the principle of absorption chromatography which is based on the interactions of two separate phase:

stationary and mobility

10

define the rate of flow Rf value of a particle solute

Rf = Dist of solute/ Dist of solvent