determination of protease acitivity

Helpfulness: 0
Set Details Share
created 3 years ago by chemistsquirrel
32 views
updated 3 years ago by chemistsquirrel
show moreless
Page to share:
Embed this setcancel
COPY
code changes based on your size selection
Size:
X
Show:
1

what enzyme is being used in this experiment?

trypsin

2

the purpose of the addition of TCA is to ______________

precipitate un-reacted substrate (protein).

3

Proteolytic enzymes catalyze the formation of proteins and peptides.

false

4

In step 2, the tybes are incubated at 37˚C for 5 minutes, the purpose is?

allow the inhibitor to inactivate the enzyme

5

The protein substrate in thsi experiment is

casein

6

For lowry's assay: the absorbance will be measured at?

600 nm

7

The lowry's assay generates a ______ color.

blue

8

functions of protease include......

  • digestion of food protein
  • clotting of blood
  • hormon production
  • regulation of other enzyme acitivities
9

The control tube contains everything except for the ________.

substrate

10

The method of determination of protease activity involves the digestion of a protein substrate by the protease followed by the quantification of base soluble peptides by Lowry's assay.

fasle

11

Alternatively, the TCA soluble peptides could be quantified by ninhydrin reagents or by measuring the absorption at ________.

280 nm