determination of protease acitivity
what enzyme is being used in this experiment?
the purpose of the addition of TCA is to ______________
precipitate un-reacted substrate (protein).
Proteolytic enzymes catalyze the formation of proteins and peptides.
In step 2, the tybes are incubated at 37˚C for 5 minutes, the purpose is?
allow the inhibitor to inactivate the enzyme
The protein substrate in thsi experiment is
For lowry's assay: the absorbance will be measured at?
The lowry's assay generates a ______ color.
functions of protease include......
- digestion of food protein
- clotting of blood
- hormon production
- regulation of other enzyme acitivities
The control tube contains everything except for the ________.
The method of determination of protease activity involves the digestion of a protein substrate by the protease followed by the quantification of base soluble peptides by Lowry's assay.
Alternatively, the TCA soluble peptides could be quantified by ninhydrin reagents or by measuring the absorption at ________.