Titration Curves of Amino Acids
a zwitterion should contain which of the following?
both negative and positive charges
which instruments will be used for today's experiment?
pH meter, magnetic stirrer, analytical balance, and buret.
what titrant is being used for the expriment?
The amino acid solution is protonated by adding 3 drops of an acid with a specific concentration. what is the acid being used to protonate the carboxyl groups on the unknown amino acid and what is the concentration of the acid?
3 M HCl
When preparing to use a pH meter, what are the normal H standards used to calibrate the pH meter?
4 red,7yellow ,10 blue
The amino acid can be titrated with either acid or base?
at it's isoelectric point, the net electronic charges of the amino acids is _________.
During titration, one needs to record the volume of the KOH with every increment of ________ pH units.
At the first equivalence point, the number of moles of KOH added is exactly equal to the number of moles of amino acid sample.
In this lab, we will identify the unknown acid by calculating it's _________.
pk1, equivalence point 1= _______
pk2, equivalence point 2 = _______
pk1 = half fully protonated, half zwitterions ( COO-)
pk2= half zwitterions, half fully deproponated ( from NH3+ to NH2)