Chapter 9 MICRO

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Microbiology
Chapter 9
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1

DNA-cutting enzymes that often form sticky ends.

Restriction enzymes

2

A self-replicating DNA molecule used as a carrier to transmit a gene from one organism to another.

Vector

3

An enzyme that links short pieces of DNA into longer pieces.

DNA ligase

4

An enzyme that links nucleotides to form DNA.

DNA polymerase

5

ii. Probably the most common vector used in genetic engineering.

Plasmid

6

The reshuffling of genes between two DNA molecules forms DNA of this type.

Recombinant

7

The kind of DNA synthesized by using mRNA as a template.

Complementary

8

DNA exchange between cells in this process uses polyethylene glycol to improve efficiency.

Protoplast fusion

9

Short segments of single-stranded DNA used to recognize a DNA sequence in a gene.

Probe

10

iii. In the blue/white screening procedure, the foreign DNA is in these colonies.

White

11

These stretches of DNA of eukaryotes do not code for proteins.

Intron

12

Sticky ends stick to each other by complementary stretches of single-stranded DNA by _________ pairing.

base

13

A collection of bacterial clones each containing a different DNA fragments is called a(n) _____________.

gene library

14

To isolate a fragment of DNA containing gene, DNA fragments of clones are separated by gel electrophoresis. This is an early step in the ____________ technique for DNA analysis.

Southern blot

15

The procedure by which billions of copies of sequence of DNA can be made in a few hours is called ___________ reaction.

polymerase chain

16

The most elegant way of introducing recombinant DNA into a plant cell is by means of ___________ plasmid, carried naturally by Agrobacterium tumafaciens.

Ti

17

The goal of the Human Proteome Project is to identify all the _________ produced by human cells.

proteins

18

The full term is restriction fragment length __________.

polymorphisms

19

___________ is the science of determining all the proteins expressed in a cell.

Proteomics

20

In Southern blotting, the gene fragments are called __________ fragment length ________.

restriction, polymorphisms

21

In the acronym RISC, the letters stand for RNA-induced __________.

silencing, complex