Microbiology: Chapter 9 MICRO Flashcards

DNA-cutting enzymes that often form sticky ends.

A self-replicating DNA molecule used as a carrier to transmit a gene from one organism to another.

An enzyme that links short pieces of DNA into longer pieces.

An enzyme that links nucleotides to form DNA.

ii. Probably the most common vector used in genetic engineering.

The reshuffling of genes between two DNA molecules forms DNA of this type.

The kind of DNA synthesized by using mRNA as a template.

DNA exchange between cells in this process uses polyethylene glycol to improve efficiency.

Short segments of single-stranded DNA used to recognize a DNA sequence in a gene.

iii. In the blue/white screening procedure, the foreign DNA is in these colonies.

These stretches of DNA of eukaryotes do not code for proteins.

Sticky ends stick to each other by complementary stretches of single-stranded DNA by _________ pairing.

A collection of bacterial clones each containing a different DNA fragments is called a(n) _____________.

To isolate a fragment of DNA containing gene, DNA fragments of clones are separated by gel electrophoresis. This is an early step in the ____________ technique for DNA analysis.

The procedure by which billions of copies of sequence of DNA can be made in a few hours is called ___________ reaction.

The most elegant way of introducing recombinant DNA into a plant cell is by means of ___________ plasmid, carried naturally by Agrobacterium tumafaciens.

The goal of the Human Proteome Project is to identify all the _________ produced by human cells.

The full term is restriction fragment length __________.

___________ is the science of determining all the proteins expressed in a cell.

In Southern blotting, the gene fragments are called __________ fragment length ________.

In the acronym RISC, the letters stand for RNA-induced __________.