Chapter 9 MICRO
DNA-cutting enzymes that often form sticky ends.
Restriction enzymes
A self-replicating DNA molecule used as a carrier to transmit a gene from one organism to another.
Vector
An enzyme that links short pieces of DNA into longer pieces.
DNA ligase
An enzyme that links nucleotides to form DNA.
DNA polymerase
ii. Probably the most common vector used in genetic engineering.
Plasmid
The reshuffling of genes between two DNA molecules forms DNA of this type.
Recombinant
The kind of DNA synthesized by using mRNA as a template.
Complementary
DNA exchange between cells in this process uses polyethylene glycol to improve efficiency.
Protoplast fusion
Short segments of single-stranded DNA used to recognize a DNA sequence in a gene.
Probe
iii. In the blue/white screening procedure, the foreign DNA is in these colonies.
White
These stretches of DNA of eukaryotes do not code for proteins.
Intron
Sticky ends stick to each other by complementary stretches of single-stranded DNA by _________ pairing.
base
A collection of bacterial clones each containing a different DNA fragments is called a(n) _____________.
gene library
To isolate a fragment of DNA containing gene, DNA fragments of clones are separated by gel electrophoresis. This is an early step in the ____________ technique for DNA analysis.
Southern blot
The procedure by which billions of copies of sequence of DNA can be made in a few hours is called ___________ reaction.
polymerase chain
The most elegant way of introducing recombinant DNA into a plant cell is by means of ___________ plasmid, carried naturally by Agrobacterium tumafaciens.
Ti
The goal of the Human Proteome Project is to identify all the _________ produced by human cells.
proteins
The full term is restriction fragment length __________.
polymorphisms
___________ is the science of determining all the proteins expressed in a cell.
Proteomics
In Southern blotting, the gene fragments are called __________ fragment length ________.
restriction, polymorphisms
In the acronym RISC, the letters stand for RNA-induced __________.
silencing, complex