Micro Lab Quiz #1

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created 3 years ago by jcalvillo883
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updated 3 years ago by jcalvillo883
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microbiology
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1

When should you wash your hands in lab?

  • In the beginning and end of lab
  • Wash if contaminated with microorganisms
2

When do you wipe down the lab bench and what do you use ?

  • Before and after each lab period
  • Disinfectant and paper towels
3

What must worn in lab?

  • Goggles, lab coats and closed toed shoes
4

What is the procedure in case of a fire or emergency evacuation?

  1. Turn off bunsen burners and hot plates
  2. Take personal items when evacuating the room
  3. Meet at the grass area near the tennis courts
  4. Don't leave before informing the instructor that you are safe
5

Where is the class meeting location outside the building in case of a emergency?

Grass area near the tennis courts

6

How do biohazard buckets look like and what are disposed in them?

  • Large buckets lined with red bags
  • Petri dishes and contaminated paper towels
7

How do slide disposal buckets look like and what are disposed in them?

  • Small bucket lined with a red bag
  • Student prepared slides and temporary slides
8

What shouldn't be placed in the slide disposal buckets?

  • commercially prepared slides
9

How should culture tubes be sorted?

  • Size
  • Type of closure
  • Tube type
10

How should culture tubes be labeled?

  • Only with Avery labels or wax pencils
11

What should be done in case of spills of liquids containing microorganisms?

  • Saturate the spill with disinfectant solution
  • Cover area with paper towels for 10 minutes
  • Put contaminated paper towels in biohazard disposal buckets
  • Repeat the the process
12

What should be done in case of spills of liquids on books, notebooks or paper?

  • Blot the spill on item
  • Place contaminated item in uv cabinet and turn the uv light for 15 min
13

What number should you call from the classroom in case of an emergency?

x4444

14

What is the objective lens magnification and total magnification for scanning lens?

  1. 4x
  2. 40x
15

What is the objective lens magnification and total magnification for low power lens?

  1. 10x
  2. 100x
16

What is the objective lens magnification and total magnification for High/dry lens?

  1. 40x
  2. 400x
17

What is the objective lens magnification and total magnification for oil immersion lens?

  1. 100x
  2. 1000x
18

What lens can immersion oil be used with?

  • 100x objective lens
19

What is the purpose of the immersion oil?

  • Forms a seal between the slide and lens
  • It is needed when using this lens to maximize the amount of light that enters the lens
  • Same refractive index as glass, so there is no diffraction (bending) of the light
20

What objective lens should be used to find the plane of focus?

  • Scanning (4x)
  • Low power (10x)
21

What two lenses can only be used with course focus?

  • Scanning
  • Low power
22

What are the first steps in the quick focusing guide?

  1. Place slide on stage using clips
  2. Using the adjustable stage knobs, to center the slide in the middle
  3. Adjust the height condenser to 1/4 inch below the stage
23

What is the second step in the quick focusing guide?

  1. Raise the stage to its highest position and place scanning or low power lens over the stage
24

What are the third steps in the quick focusing guide?

  1. Lower the stage using coarse focus knob
  2. Sharpen focus using fine focus knob
  3. Adjust light using intensity knob and iris diaphragm lever
25

What are the fourth steps in the quick focusing guide?

  1. To increase the magnification, rotate next magnification lens
26

When rotating to a higher magnification, what shouldn't and should do?

  • Shouldn't drop the stage between objective lenses
  • Should just adjust fine focus and reposition iris diaphragm for correct light
27

What does ocular mean?

...

28

What does objective mean?

...

29

What does refraction mean?

...

30

What is a compound microscope?

...

31

What is the fine focus?

...

32

What is the course focus?

...

33

What is the condenser lens?

...

34

What is the iris diaphragm?

...

35

What is the working distance?

...

36

What is the field diameter?

...

37

When should lens paper and kimwipes be used?

...

38

What is the eyepiece head?

...

39

What are three ways to adjust the light of the microscope?

  1. Using the light intensity knob
  2. Iris diaphragm knob
  3. height of condenser
40

How should you carry the microscope?

  • Carry with both hands
  • One on base and one holding the arm
  • Don't support using the stage
41

You can rotate the eyepiece head. True or False

False

42

What should you make sure before placing prepared and stained slide on stage of microscope?

  • Slide has to be completely dry and no stain remains on the underside of the slide
43

You can clean the lenses of the microscope with kimwipes. True or False

False

44

What should be the position of the microscope when returning it?

Arm is facing outward and eyepieces is facing inward

45
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What is A?

Eyepieces

46
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What is B?

Eyepiece head

47
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What is C?

Headpiece holding screws

48
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What is D?

Arm

49
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What is E?

Power switch

50
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What is F?

Light intensity knob

51
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What is G?

Adjustable stage knobs

52
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What is H?

Fine focus knob

53
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What is I?

Light source

54
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What is J

Condenser

55
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What is K?

Iris diaphragm lever

56
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What is L?

Stage

57
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What is M?

Slide clip

58
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What is N?

Objective

59
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What is O?

Revolving nosepiece

60
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What is A?

Condenser height knob

61
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What is B?

Fine focus knob

62
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What is C?

Coarse focus knob

63

Which groups of microorganisms can be grown in artificial media and which cannot?

  1. Bacteria and fungi can be grown in artificial media
  2. Viruses cannot be
64

What is agar used for?

It is solidifying agent for media

65

Where does agar come from?

  • Seaweed
  • does not provide any nutritional value for bacteria
66

Which temperature does agar become a liquid?

100 C

67

Which temperature does agar become a solid?

40 C

or 45 - 50 C

68

What is NB?

Nutrient Broth for

69

What is NA?

Nutrient Agar

70

What is TSA?

Tryptic soy agar

71

What is the visual clue that can determine whether agar is solid or liquid?

  • Clear - Liquid
  • Opaque - Solid
72

What does ubiquity mean?

The ability of microorganisms to be found everywhere at the same time

73

What is normal flora and give some examples?

  • Microorganisms that colonize a host without causing a disease under normal conditions
  • Staphylococcus epidermidis
  • Streptococcus mitis
  • Spirochetes
74

What is a pathogen and give some examples?

  • a disease-causing organism
  • Salmonella
  • Listera
  • Staphylococcus saprophyticu
75

Conversion factor of L to mL

1 L = 1000 mL

76

Conversion factor of g to mg

1 g = 1000 mg

77

cm = ?

1/100 m

78

mm = ?

1/1000 m

79

(micron) um = ?

1/106 m

80

What is the purpose of autoclaving media before use?

  • autoclave carries out that exact function of sterilizing materials. It is a machine that uses pressure and steam to reach and maintain a temperature that is too high for any microorganisms or their spores to live. Autoclaving is used to kill all micro-organisms that are in the culture. A clean culture free of all micro-organisms is essential as you only want the organism of intrest to grow.