Ch 3

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Microbiology
Chapter 3
updated 2 years ago by mrsperez789
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1

BACTERIA ARE LARGER THAN VIRUSES

*SMALLEST TO LARGEST*

OXYGEN ATOM

WATER MOLECULE

COLD VIRUSES

BACTERIA

SKIN CELL

STATUE OF LIBERTY

2

SIMPLE STAINS

-BACTERIA CELLS ARE NEGATIVELY CHARGED

-BASIC DYE COLORS THE CELL AND IS CALLED A SIMPLE STAIN

-ACIDIC DYE COLORS THE BACKGROUND

-A MORDANT IS A CHEMICAL THAT INTENSIFIES THE STAIN

3

DIFFERENTIAL STAINS

-STAINS CAN COLOR DIFFERENT BACTERIA DIFFERENTLY (KIND OF LIKE HAIR COLORING'S COLOR DIFFERENT HAIR COLORS DIFFERENTLY)

-THESE DIFFERENCES HELP US DISTINGUISH DIFFERENT ORGANISMS

-THE TOW MOST COMMON DIFFERENTIAL STAINS ARE GRAM STAINS AND ACID-FAST STAINS

4

GRAM STAIN

GRAM + BACTERIA WILL BE PURPLE

GRAM - BACTERIA WILL BE PINK

5

4 STEPS

PRIMARY STAIN- CRYSTAL VIOLET

MORDANT - IODINE

DE-COLORIZING AGENT- ALCOHOL/ACETONE

COUNTER STAIN- SAFRANIN

6

4 STEPS

*GRAM POSITIVE CELLS

CRYSTAL VIOLET- PURPLE

IODINE- PURPLE

ALCOHOL/ACETONE- PURPLE

SAFRANIN- PURPLE

7

4 STEPS

*GRAM NEGATIVE CELLS

CRYSTAL VIOLET- PURPLE

IODINE- PURPLE

ALCOHOL/ACETONE- COLORLESS

SAFRANIN- RED