Activity 5: Determining Hemoglobin Concentration

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1

1. Can a person be anemic even with a normal RBC count?

2. Why?

1. Yes.

2. The total hemoglobin concentration for the RBCs may be low.

2

What RBC protein is responsible for oxygen transport?

Hemoglobin

3

What is the most accurate way of measuring the oxygen-carrying capacity of the blood?

Determine the hemoglobin content

4

How is oxygen transported by RBCs?

Oxygen combines reversibly with the heme (iron containing portion) of the hemoglobin protein. It is then picked up by the RBCs in the lungs and unloaded throughout the body.

5

What is the relationship between the number of hemoglobin molecules and and oxygen transportation?

The more hemoglobin molecules there is, the increased amount of oxygen transported.

6

Normal blood contains what range of hemoglobin?

12-18 g of hemoglobin per 100 ml of blood

7

Compare the hemoglobin content in men to women.

Men: 13-18 g of hemoglobin per 100 ml of blood

Women: 12-16 g of hemoglobin per 100 ml of blood