Activity 1: Identifying the parts of a Microscope
How to hold a microscope when transporting it?
Hold it upright with one hand on the arm and the other supporting the base.
What to use to clean the lens?
Grit-free lens paper.
What objective should you begin focusing with?
When should you ONLY use the coarse adjustment knob?
With the lowest-power lens (scanning-4x and low power-10x)
T/F: Always use a coverslip with wet mount preparations.
A microscope must be stored in what 4 specific conditions?
1. No slide on the stage.
2. Lowest power objective in position.
3.Cord wrapped around the base.
4.Dust cover covering the microscope.
Function of the base?
Provides a flat surface to support the microscope.
Function of the substage light?
Passes the light from the lamp upward to the objective lens.
Function of the light control knob?
Adjust the intensity of the light passing through the specimen.
Function of the mechanical stage?
Holds the slide for viewing.
Function of the mechanical stage knobs?
Allows position readjustment of the slide, moving it forward, backwards, right, or left.
Function of the condenser?
Concentrates light on the specimen.
Function of the iris diaphragm lever? Closing the diaphragm improves what?
Adjusts the amount of light passing through the condenser. Closing the diaphragm improves contrast.
Function of the coarse adjustment knob?
Adjust the height of the stage to focus the specimen.
Function of the fine adjustment knob?
Precise focusing once the course focus has been completed.
2 functions of the head?
1. Attaches to the nosepiece to support the objective lens.
2. Provides attachment of the eyepieces and ocular lens.
Function of the arm?
Connects the base and the head.
Function of the nosepiece?
Carries the objective lenses and permits the changing between them.
Function of the objective lenses? What are the magnification of the four objectives?
Magnifies the specimen. Scanning: 4x, Low-power: 10x, High-power: 40x, and Oil immersion: 100x.
Function of the ocular lenses?
Magnifies the specimen 10x.
Function of the on/off switch?
Turns on/off the light.
The objective lens magnifies the specimen to produce a what?
The real image is then magnified by the ocular lens to produce a _____ that your eyes see.