Microbiology Test 1 Flashcards


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1

Putting your loop in the flame and decontaminating the table top is an example of what?

aseptic technique

2
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This is an example of?

streak plating

3

The study of life to small to see without a microscope is called?

microbiology

4

What are the six type of organisms studied in microbiology?

bacteria, archae, fungi, protozoa, algae, and viruses

5

What are the fields associated with bacteria, algae, fungi, protozoa, parasites, and viruses in that order?

bacteriology, phycology, mycology, protozoology, parasitology, and virology

6

What are the 3 domains and which one are we in?

Bacteria, archae, eukarya. Plants and animals are in eukarya.

7

What does eukaryote mean literally? Prokaryote?

eu=true, pro=before, karyote=nucleus

8

What are the five kingdoms?

animals, plants, protists, fungi, monera

9

The kingdom plants includes?

plants and multicellular algae

10

The kingdom protists includes?

protozoa and unicellular algae

11

The kingdom monera includes?

eubacteria and archaeobacteria
(true bacteria and ancient bacteria)

12

What are the five I's?

innoculation, incubation, isolation, inspection, identification

13

What does TSA stand for?

tryptic soy agar

14

How many cells in our bodies are bacteria?

9/10

15

Human cells are much ____ than bacteria?
(larger or smaller)

larger

16

How many species of bacteria live in the human eye?

5

17

How many species of bacteria live in the mouth?

600

18

How many species of bacteria live in the intestines?

400 (3 lb. of bacteria)

19

How many species of bacteria live on men's hands? Women's hands?

120, 180

20

What is a general purpose medium?

one in which all or most species can grow

21

What is a selective medium?

one in which only one species grows

22

What is a general purpose nondifferential medium?

one where all species have a similar appearance

23

What is a differential medium?

one where all 3 species grow, but may show different reactions

24

What are the steps of Gram staining?

1.Make a slide by putting 1 drop of water, put bacteria into water, let dry, then run through flame 4 or 5 times.
2.Crystal violet for 20 sec.
3.Wash for 2 sec.
4.Gram's iodine for 1 min.
5.Decolorize with alcohol for 10-20 sec.
6.Wash for 2 sec.
7.Safranin for 1 min.
8.Wash for 2 sec.
9.Blot dry.

25

Gram negative is what color? Gram positive?

red, blue

26

Round, spherical shaped bacteria are categorized into what?

cocci

27

Long cylindrical shaped bacteria are categorized into what?

rods

28

What is the genus and species of the bacteria that caused a man's towels to turn red and death in termites?

Serratia marcescens (UNDERLINE, BOLD, OR ITALICIZE THIS!!!)

29

Name the bacteria that is gram negative rods, grows in cetrimide agar, and has a distinct yellow or green/blue color that glows under a blacklight. It also makes blue pus in infected burn victims.

Pseudomonas aeruginosa

30

Who was responsible for Gram staining?

Hans Christian Gram

31

What part of the body did Gram stain bacteria in when he developed Gram staining?

lungs, he was looking for bacteria that caused pneumonia and found Streptococcus pneumonia

32

Should one use new cells or old cells for Gram staining?

new, old cells lose the ability to hold stain because their cell walls have weakened

33

You are doing a Gram stain and accidentally over heat the cells in the flame. Do you think this could mess up your results?

Yes, excessive heating disrupts the cell walls.

34

What are the 4 basic steps of Gram staining?

1.primary stain
2.mordant
3.decolorization
4.counterstain

35

If your bacteria can swim it is called what?

motile

36

When looking for spores, should you use old or new cultures?

Old, most bacteria that produce spores produce them after a given time instead of right at the beginning.

37

Where can an endospore be located in a bacteria cell?

terminal, subterminal, central

38

The most resistant form of life is a what?

spore

39

Anaerobic bacteria grows how?

without oxygen

40

Aerobic bacteria grows how?

with oxygen

41

Facultative bacteria grows how?

with or without oxygen

42

What is the reference book used to look up bacteria called?

Bergey's Manual of Systematic Bacteriology
vol.1=gram negative
vol.2=gram positive

43

What are the 4 divisions of bacteria?

-Gracilicutes (gram negative mucopeptide (peptidoglycan) wall)
-Firmicutes (gram positive " " wall)
-Tenericutes (lack a cell wall)
-Mendosicutes (have a non mucopeptide wall)

44

What is a strain?

identical descendants of a single bacterial cell

45

Do strains result from mutations?

most likely, yes

46

What is the ATCC?

they keep type species of strains

47

What are the different types of strains?

biotypes, serotypes, pahtotypes, phagotypes, morphotypes

48

H antigens are for? O antigens? K antigens?

flagella, cell wall, capsule

49

What are biotypes?

bacteria strains that differ in some special biochemical or physiological property

50

What are serotypes?

bacteria strains that differ based on antigens for the cell body, flagella, or capsule

51

What are pathotypes?

bacteria strains that differ in whether they are dangerous to life
(ex. pathogenic E. coli)

52

What are morphotypes?

Bacteria that are the same species but different strains that look different on a medium.

53

What are phagetypes?

Bacteria strains that differ in their resistance to viruses.
(ex. phagetype 80/81/15/3)

54

What type of medium is blood agar?

differential medium

55

What does bacteriophage mean?

"bacteria eating"

56

What is a bacteriophage?

a virus that attacks SPECIFIC bacteria

57

A _____ attaches to a cell wall and injects its DNA, where it replicates itself. When there are too many of these in the cell, it will burst open the cell with several copies.

virus

58
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What are the clear spots in the bacteria lawn?

viral plaques

59

How many versions of E. coli O antigens are there?

173

60

How many versions of the E. coli H antigens are there?

60

61

What is O157:H7?

E. coli cell wall version 157 and flagella version 7. It is harmful and causes hemorrhage of intestines.

62

What does ATCC stand for?

American Type Culture Corporation

63
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What test is this an example of?

capsule stain

64
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Know these!

ok

65
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Would this bacteria test positive for spores?

Yes