Lab manual: Urease Test

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Microbiology
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Lab manual Urease test Microbiology 205
updated 9 years ago by jenkarmata
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College: Second year
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1

Urease is produced by some microorganisms, is an enzyme that is especially helpful in identification of

Proteus vulgaris.

2

Urease is produced by some

microorganisms

3

Although other organisms may produce urease, their action on the substrate urea tends to be slower than that seen w/

Proteus species.

4

Other organisms my produce urease, but their action on ______ ____ tends to be slower than that seen with Proteus species.

substrate urea

5

Is urease an enzyme?

~Yes~

6

The urease test serves rapidly to distinguish members of this genus from other...

non lactose-fermenting enteric microorganisms.

7

Whay type of enzyme is Urease?

Hydrolytic

8

What is the function of Urease (hydrolytic enzyme)

Attack nitrogen & carbon bond in amide compounds such as urea & forms the alkaline end product AMMONIA.

9

What attacks nitrogen & the carbon bond in amide compounds such as urea & forms the alkaline end product ammonia?

Urease (which is a hydrolytic enzyme)

10

Urease attacks _a___ & the _b_______ in __c______ such as urea & forms the alkaline end product __d_____.

a. nitrogen
b. carbon bond
c. amide compounds
d. ammonia

11

The presence of urease is detectable when the organisms are grown in a urea broth medium containing the pH indicator

phenol red

12

As urea is split into it products, the presence of __a___ creates an alkaline environment that causes the the phenol red to turn a deep __b___

a. ammonia
b. pink

13

The presence of ammonia turning the phenol red a deep pink is a ___a_____ reaction for the presence of __b_______.

a. positive
b. urease

14

On a urease test the color yellow means

Negative reaction for the presence of urease.

15

On a urease test the color pink means

Positive reaction for the presence of urease.