Microbio Lab UV Light

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created 4 years ago by Michele_Schweers_Simms
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1

Most effective germicidal wavelength of UV light

256nm

2

Benefits of UV light as a microbial agent

effective, cheap and quick

3

Disadvantages of UV light as a microbial agent

Damages human skin and eyes and easy to block because it has a poor penetrating power.

4

Ultraviolet light primarily affects the cell's

DNA

5

The wavelengths of light effective in light-repair mechanisms

range of 420-540nm

6

Name three places where UV light is used for germicidal properties

Air systems of hospitals, surgical tools and food industry

7

One cause of failure of DNA to replicate when irradiated with UV light is the formation of these

thymine dimers; dimers between adjacent thymine molecules

8

Even though some organisms are able to repair UV light damage, surviving cells often carry

mutations

9

In addition to light-medicated photoreactivation, repair may take place as

dark-mediated repair.

10

Photoreactivation

the light repair system and results from an enzyme called DNA-photolyase

11

Name 3 factors that limit the use of UV light as a sterilizing agent

poor penetrating power, effectively blocked out by opaque surfaces and only useful with direct exposure