Lab Practical #2

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created 6 years ago by Sharleezybby
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updated 6 years ago by Sharleezybby
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1

Amylase

Hydrolyzes starch; releasing free glucose

2

Positive for starch hydrolysis

Colorless around growth

3

Negative for starch hydrolysis

Purple around growth

4

DNase

Hydrolyzes DNA; releasing free nucleotides

5

Positive for DNase hydrolysis

Clear around surrounding the growth

6

Negative for DNase hydrolysis

Green area surrounding the growth

7

Collagenase

Hydrolyzes gelatin; releasing free amino acids

8

Positive for collagenase--Fast gelatin hydrolysis

Liquid after 24-48 hrs.

9

Positive for collagenase--Slow hydrolysis

Liquid after 1 week

10

Continue to incubate up to 1 week

Solid after 24-48 hrs.

11

Negative for collagenase; no gelatin hydrolysis

Solid after 1 week

12

Mannitol

Fermentable carbohydrate (sugar alcohol) that some pathogenic Staphylococci can convert to acidic end-products

13

Positive for mannitol

Growth & medium turned yellow

14

Negative for mannitol

Growth & medium did not turn yellow

15

Coagulase

Enzyme that participates in clotting blood plasma

16

Positive for coagulase

Rabbit plasma is coagulated

17

Negative for coagulase

Rabbit plasma is NOT coagulated

18

Novobiocin

Antibiotic that can be used in the differentiation of Staphylococcus species

19

Positive for Novobiocin (susceptible)

> 16 mm

20

Negative for Novobiocin (resistant)

< 16 mm

21

Catalase

Enzyme that degrades hydrogen peroxide to water & oxygen gas

22

Positive for catalase

Bubbles

23

Negative for catalase

No bubbles