micro lecture test #2

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1

Which one of the following is a method of vertical gene transmission?
a) Cell division
b) Transduction
c) Transformation
d) Conjugation

Cell division

2

Which of the following requires cell-to-cell contact?
a) Conjugation
b) Transduction
c) Transformation
d) Mutation

Conjugation

3

When the antibiotic chloramphenicol binds to the 50S portion of the ribosome between the A and P sites, the effect is to:
a) prevent transcription.
b) prevent attachment of tRNA.
c) prevent peptide bond formation.
d) stop the ribosome from moving along the mRNA.

prevent peptide bond formation.

4

Which one of the following nucleotide sequences is most likely affected by ultraviolet light?
a) AGTTTC
b) GATCAA
c) TATATA
d) GAAACC

AGTTTC

5

The DNA fragment 3´ CGAATCA will most likely hybridize with:
a) 3´ CGAATCA
b) 3´( GCAATGT
c) 5´ GCUUAGU
d) 5´ CGTTAAG

5´ GCUUAGU

6

Which of the following enzymes and their function is mismatched?
a) RNA polymerase-makes DNA
b) DNA ligase-joins pieces of DNA
c) DNA polymerase-makes DNA
d) DNA polymerase-proofreads DNA

RNA polymerase-makes DNA

7

In 1928, in his discovery of transformation, Griffith did not know that:
a) DNA from Smooth cells could transform Rough cells.
b) Streptococcus pneumoniae causes disease.
c) Rough cells do not cause disease.
d) Smooth cells can cause disease.

DNA from Smooth cells could transform Rough cells.

8

The antibiotic kasugamycin blocks binding of tRNA-Fmet. From this information you can conclude that kasugamycin prevents:
a) polypeptide initiation in prokaryotes.
b) mRNA-ribosome binding in eukaryotes.
c) mRNA-ribosome binding in prokaryotes
d) polypeptide elongation in eukaryotes.

polypeptide initiation in prokaryotes.

9

The following steps are required for DNA replication. What is the second step?
a) DNA ligase joins DNA fragments
b) DNA polymerase removes the RNA primer
c) an RNA primer is synthesized
d) a replication fork forms

an RNA primer is synthesized

10

Your lab partner has mixed a dead tryptophan+ strain of Bacillus subtilis with a live tryptophan- strain and observes that her B. subtilis culture is now tryptophan+. The most likely explanation for this is:
a) transformation.
b) conjunction.
c) transduction.
d) mutation.

transformation.

11

Which of the following is not true of translation?
a) A single mRNA may have several ribosomes attached.
b) A molecule of tRNA can bind to both an mRNA molecule and an amino acid.
c) Each amino acid is coded for by a single codon.
d) Three different nonsense codons code for termination of protein synthesis.
e) The "language" of nucleotides is changed to the "language" of amino acids.

Each amino acid is coded for by a single codon.

12

Which of the following is not true of DNA replication?
a) Each strand of the parent DNA serves as a template for a newly synthesized complementary strand.
b) DNA polymerase is required to add new nucleotides to the growing end of the new DNA strand.
c) DNA ligase joins the small DNA fragments of the lagging strand.
d) In E. coli, replication proceeds bidirectionally from the origin of replication.
e) RNA polymerase is not involved in the process.

RNA polymerase is not involved in the process.

13

What was primary observation of Griffith's 1928 work using Streptococcus pneumoniae ?
a) He observed that avirulent streptococci became virulent after transformation.
b) He was surprised to learn that live, virulent streptococci could kill mice.
c) He found that DNA was the genetic material of the cell.
d) He observed that DNA could be transferred between living bacteria through conjugation.
e) He determined the mechanism of transformation.

He observed that avirulent streptococci became virulent after transformation.

14

For the peptide with the amino-acid sequence, proline-alanine-glycine (pro-ala-gly), the DNA template strand could have the sequence:
a) GGC CGA CCG.
b) CCU GCC GGC.
c) CGC GCC GGA.
d) GGU CGA CCA.
e) CCG GCC GGG.

GGC CGA CCG.

15

Transformation:
a) requires a virus to inject the donor DNA.
b) appears to occur in nature for most bacteria at most times.
c) results in the transfer of a complete set of genetic information from donor to recipient.
d) requires a recipient cell to be "competent."
e) requires a conjugative plasmid

requires a recipient cell to be "competent."

16

Genes whose products are produced constantly are

constitutive.

17

The process by which normally repressed genes are "turned on" is referred to as

induction.

18

The codons UAA, UAG, and UGA signal the end of a gene sequence and are known as _______ codons.

...

19

Genome

The entire genetic makeup of an organism (ALL ITS DNA

20

Genotype

The makeup of specific genes

21

Phenotype

The expression of those genes

22

Replication

DNA POLYMERASE
read the bases and add complementary bases
Semi conservative which means each new DNA has one new and one old Strand

23

Transcription

RNA polymerase binds to promoter
2). DNA unwinds
3). RNA polymerase reads and synthesizes RNA

24

translation

Ribosomes bind to mRNA (start codon)
2). Two sites on bacterial ribosomes, P&A
3). tRNA bonds to start codon