Microbiology Lab Final Review

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1

Eosin Methylene Blue Agar

  • It contains the dyes eosin and methylene blue, which inhibit the growth of gram-positive bacteria and therefore select for gram-negative bacteria.
  • Strong acid production by E. coli precipitates
2
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What kind of streak plate is this?

eosin methylene blue

3
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What kind of streak plate is this?

Casein Agar

4
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Nutrient agar plate?

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Right side

5
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Citrate Test

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A) (-)
B) (+)

6
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Starch Hydrolysis

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Left:(-)
Right: (+)

7
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Casein Hydrolysis

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Top: (-)
Bottom: (+)

8
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Gelatin Hydrolysis

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A) (+)
B) (-)

9
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Catalase test

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A) (-)
B) (+)

10

What test is this?

Lactose

11
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What test is this?

Glucose

12
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What test has a red top?

INC

13

Acid Fast Stain Procedure (pg.92)

1. Carbolfuchsin-->Heat fix (5 min)
2. Acid Alcohol (1 min)
3. Methylene Blue (1 min)

14

Gram Stain Procedure (pg.87)

1. Crystal violet (1 minute)
2. Iodine (1 minute)
3. Alcohol (1 minute)
4. Safranin (1 minute)

15

Endospore Stain Procedure (pg.99)

1. Malachite Green over steam (10 min)
2. Water
3. Safranin (30s)

16

Nitrate Reduction Test

I N C