Exercise 5-6 Micro Lab: Oxidase Test
glucose is oxydized in ____
after oxydation what happens to glucose in glycolysis?
it is converted into two molecules of pyruvate and reduces two NAD to NADH
what happens to the pyruvate?
each pyruvate is oxidized and converted to a two carbon molecule called acetyl CoA and one molecule of CO2 which reduces another NAD and NADH
What happens in the KREBS cycle?
two more molecules of CO2 are produced per Acetyl CoA and reduces three more NADs and one FAD to FADH2.
What is the job of the electron transport chain?
to convert all the reduced coenzymes back to the oxidized state
2 functions of the electron transport chain
1. transport electrons down a chain of molecules with increasingly positive reduction potentials to the terminal electron acceptor
2. generate a proton motive force by pumping H+ out of the cell thus creating an ionic imbalance that will drive the production of ATP by way of membrane ATPases
_______, ________, and __________ are important ETC molecules unable to donate protons
The last enzyme in the ETC, in Complex IV, is called _______
chromogenic reducing agent
chemicals that develop color as they become oxidizes
what does cytochrome c oxidase have the ability to do?
to oxidize cytochrome c and catalyze the reduction of cytochrome c by a chromogenic reducing agent