Exercise 5-12 Micro Lab: Starch Hydrolysis
reactions that use water to split complex molecules
intracellular enzymes detected by a Urease Test that are required to catalyze hydrolysis reactions
when the enzyme catalyzes its reaction inside the cell
enzymes secreted from the organism to catalyze reactions outside the cell
an exoenzyme that liquefies the solid medium
Nutrient geletain detects what?
What 4 media are plated media that identify extracellular enzymes capable of diffusing into the medium and producing a distinguishable halo of clearing around the bacterial growth?
1. DNase Agar
2. Milk Agar
3. Tributyrin Agar
4. Starch Agar
a polysaccharide made up of a-D-glucose subunits.
Starch exists in what two forms?
1. linear (amylose)
2. branched (amylopectin)
usually as a mixture with the branched configuration being predominant
Whats the deal with starch and the bacterial cell membrane?
Starch is too large to pass through it, so it has to split into smaller fragments or individual glucose molecules in order to be of any metabolic value
these enzymes are able to hydrolyze starch by breaking the glycosidic linkages between sugar subunits.
a simple plated medium of beef extract, soluble starch, and agar
What is used to detect the presence or absence of starch in the vicinity around the bacterial growth?
Iodine reacts with starch and produces what color?
a blue or dark brown color
starch agar was originally designed for cultivating _____
a clearing around the bacterial growth indicates what?