Exercise 2-12 Micro Lab: Steam Sterilization

Helpfulness: 0
Set Details Share
created 5 years ago by OceansVoice
135 views
updated 5 years ago by OceansVoice
show moreless
Page to share:
Embed this setcancel
COPY
code changes based on your size selection
Size:
X
Show:
1

germicides

substances or systems, chemical or physical, used to prevent the spread of pathogens

2

broad-spectrum

targeting a wide variety of pathogens

3

3 categories germicidal systems fall into

1. decontamination
2. disinfection
3. sterilization

4

decontamination

reduction of pathogenic microorganisms to a level at which items are safe to handle without protective attire

5

__________ is the lowest level of control

decontamination

6

Decontamination usually includes what? (2)

1. physical cleaning with soaps or detergents
2. removal of all (ideally) or most organic and inorganic material

7

_______ is considered the critical first step toward disinfection or sterilization because _______

proper cleaning of all instruments and surfaces

to be fully effective, a disinfectant or sterilant must come in direct contact with all pathogens present. materials left to dry on a surface or apparatus can actually shield pathogens from a disinfecting or sterilizing agent or otherwise neutralize it.

8

disinfection

kills large numbers, if not all, of the targeted pathogens but typically do not kill large numbers of spores

9

Why are some disinfectants called chemical sterilants?

because they have the ability to kill all vegetative cells and spores

10

sterilization

complete elimination of viable organisms including spores and, as such, is the highest level of pathogen control

11

6 methods of disinfection

1. liquid chemical agents
2. solids
3. gasses
4. dry heat
5. moist heat
6. ultraviolet light

12

9 methods of sterilization

1. chemical
2. gasses
3. incineration
4. dry heat
5. moist heat
6. ethyline oxide gas
7. ionizing radiation
8. low temperature plasma
9. low temperature ozone

13

3 types of ionizing radiation

1. gamma
2. x-ray
3. electron-beam

14

low temperature plasma

utilizing a combination of chemical sterilants and ultraviolet radiation in a vacuum chamber

15

low temperature ozone

utilizing bottled oxygen, water, and electricity in a chamber

16

_____ is the most effective and most common means of sterlization

steam

17

autoclave

the device used for steam sterlization

18

How does an autoclave work?

it uses super heated steam under pressure to kill heat resistant organisms

19

3 examples of heat resistant organisms

1. Bacillus
2. Geobacillus
3. Clostridium

20

Sterilization temperature is usually set between _____ and _____

121-127 degrees Celsius

21

How long to steam?

15 min

22

biological indicator

test systems that contain something living.

the only way to make sure that sterilization is complete

23

one way of telling if sterilization is complete is through ______________

color coded autoclave tape (grey is autoclaved, blue is not autoclaved)

24

bacterial spores

the dominant form of an organism that is highly resistant to both chemical and physical means of control

if an autoclave kills these, it is safe to say it will kill everything else microbially

25

A vial turns from _______ color to ______ color when it is autoclaved. What do the two colors mean?

yellow to purple. yellow is not autoclaved (evidenced by blue band of color strip too) and fermentation/acid production is in the medium. the organism is alive. The purple is autoclaved (evidenced by grey strip) and there is no fermentation/acid production in the medium. the organism is dead.