Compound Light Microscope Parts (Definitions)

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created 7 years ago by Neil
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Define key terms for the Microscopy Lab. Includes different types of Microscopes, parts of compound light microscope etc..
Grade levels:
College: First year
Subjects:
bio 101 lab
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1

Body

connects oculars(eyepieces) to nosepiece

2

Nosepiece

rotates to change objectives lenses used to magnify specimen

3

Objective Lenses

scans the specimen

4

Scanning Objective

holds 4X lens

5

Low Power Objective

holds 10X lens

6

High-Dry Power Objective

holds 40X lens

7

Oil Immersion Objective

holds 100X lens

8

Total Magnification

ocular magnification X objective magnification

9

Stage

holds and supports microscope slide/specimen

10

Diaphragm

regulates the amount of light used to view the specimen

11

Condenser

focuses light rays on the specimen

12

Illuminator

contains and shines light through the specimen

13

Base

supports instrument (stage and arm)

14

Oculars (Eyepiece)

series of lenses to look through to view specimen
holds 10X lens

15

Arm

connects the base to the body, allow user to carry instrument

16

Stage Clip

lever that anchors the slide in place

17

Coarse Adjustment Knob

used for initial focusing on scanning power objective lens

18

Fine Adjustment Knob

used for final focusing

19

Slide Position Adjustment Knobs

adjusts the position of the slide

20

Light Microscope

form images using lenses and wavelengths of light in the visible spectrum

21

Compound Light Microscopes

used to view thin sections of specimens or live microscopic organism

22

Stereo-dissecting Light Microscopes

used to view the external surfaces of large specimens

23

Electron Microscopes

used to view specimens and non-living cells at magnifications up to 300,000X

24

Scanning Electron Microscopes

used to view the external surfaces and cells in three dimensions

25

Transmission Electron Microscopes

used to study the internal surfaces of cells

26

Magnification

is how much larger a specimen appears compared to its actual size

27

Resolution

is the extent to which two structures can be distinguished from one another

28

Contrast

is the degree to which an image stands out against its background

29

Ways to increase contrast

a) the use of dye/stains
b) adjustments to the diaphragm of the microscope