Micro Lab: Streak and Spread Swab Techniques
What is the goal of creating a spread plate?
To distribute bacteria so that a uniform layer of growth is present across the entire plate.
What are spreads commonly used for?
antibiotic and disinfectant testing
What is necessary in order for the spread tests to be done in a uniform manner?
the amount of bacteria inoculated onto a spread plate must be the same from plate to plate
How do we achieve uniformity from plate to plate in spread tests?
by creating solutions with the same turbidity
a measure of how cloudy or hazy a solution is as a result of bacterial growth
This standard is an inert solution with a set turbidity.
What is the purpose in creating a bacterial solution with the same turbidity as the standard?
We will be applying a known and consistent concentration of bacteria to all plates