Components of a bacteriophage (4)
1. icosahedral
2. DNA
3. Tail fibers
4. base plate
diploid cell cultures and continuous cell cultures differ in
longevity and source of cells
prisons are different because they lack
nucleic acids
in virus taxonomy, some virus families are derived from the name of
an important member in the family
Prusiner discovered
prions
Lytic Replication cycle:
earliest to latest (5)
Attachment, Entry, Synthesis, Assembly, Release
majority of cases of infant diarrhea are caused by _______ virus
dsRNA
zones of clearing cell cultures which are a result of virus infection are called _________ which are infected with ___________, which might be caused by a _________ viral infection
plaques, bacteriophage, lysogenic
lipid membrane is present in both ______ and _____
cells, viruses
double stranded RNA genomes can be found only in ________
viruses
(3) are associated with the attachment of a bacteriophage to a bacterial cell
1. random collisions
2. chemical attractions
3. receptor specificity
many diseases of plants are caused by infectious RNA molecules lacking ____
capsids
most viruses cannot be seen by ___________ microscope
light
during ____, viruses remain dormant in a cell
latency
viruses are shed slowly and steadily during _________ infection
persistent
virus replication results in the ____ of the cell in a lytic
death
cytoplasm is a characteristic of ________ only
cells
proteins are present in both _________ and _________
cells, viruses
(2) membranes that can give rise to a viral envelope
nuclear and cytoplasmic membrane
host specificity of a virus is due to interactions between _______ and _______ surface molecules
viral, cellular
tumors invade other organs and tissues through process called
metastasis
the phenomenon of transduction is associated with
assembly
the outermost layer of a virion fulfills (2)
protection and recognition
scientist- was the first person to demonstrate the existence of viruses
Ivanowsky
double stranded DNA genomes are found in
both cells and viruses
T/F
viroids have protein in their structure
FALSE, they viroids do not have protein in their structure
________ is a mechanism of release for enveloped viruses
budding
viroids infect ________
plants
acelluar-
disease causing agents
Acellular lack _______,
cannot __________, _________, _______ to their environment
lack CELL STRUCTURE
cannot METABOLIZE, REPRODUCE, GROW
RESPOND to their environment
Acellular includes (3)
viruses, viroids, prions
Virus-
tiny infectious agent
virus has a _______ which is surrounded by a proteinaceous ________ that form a coat called _______
virus has a NUCLEIC ACID, which is surrounded by a proteinaceous CAPSOMERE, that forms a coat called a CAPSID
virus can be ______ extracellular state and intracellular state
both
virion is a complete _________ which includes a _____ and a ________ outside of the cell
viral particle
includes a nucleic acid and a capsid
the genomes of viruses include either
DNA or RNA
primary source of genomes classification
DNA or RNA
what is a virus that infects a bacterial cell?
bacteriophage (phage)
virions can have a membranous _______ or be naked w/o
envelope
the outermost layer of a virion fulfills _____ and _______ functions of the virus
protection, recognition
viruses are classified based on (4)
1. presence of an envelope
2. nucleic acid
3. shape
4. size
viruses depend on random contact with a _______ for replication
specific host cell type
Lytic Replication Cycle (5) stages
1. attachment
2. entry
3. synthesis
4. assembly
5. release
once attachment has been made between virion and host cell:
with phages only the ________ enters the host cell.
With animal viruses the ___________ virion often enters the cell
nucleic acid, entire virion
removal of capsid is a process called
uncoating
within the host cell, the viral nucleic acid directs _________ of more viruses using _______ and _____ of the host cell
synthesis, enzymes and ribosomes
assembly of synthesized virions occur in the
host cell
when virions are released from the host cell either called _____ of the host cell or by extrusion of envelope virions through host cytoplasmic membrane called _______
lysis, budding
what is it called when bacteriophages enter a bacterial cell and remain inactive?
temperate (lysogenic)
inactive phages are called
prophages
___________ results when phages carry genes that alter the phenotype of a bacterium
lysogenic conversion
process where an animal virus remains inactive in a cell
latency
latent virus is also known as a
provirus
with the exception of B virus, ______ viruses act like cellular DNA in transcription and replication
dsDNA
retroviruses are
+ssRNA viruses that carry an enzyme reverse transcriptase
_______ is uncontrolled cellular reproduction in a multicellular animal
neoplasia
tumor is harmless
benign
tumor is invasive//cancerous
malignant
term that describes the spreading of malignant tumors and invading other organs and tissues
metastasis
viruses must be cultured inside whole organisms in chicken eggs or cell cultures because viruses cannot
metabolize or replicate ALONE
when a mixture of bacteria is grown on an agar plate, bacteria infected w phages lyse, producing clear areas are called
plaques
a technique called _______ enables the estimation of phage numbers
plaque assay
viruses can be grown into 2 types of cell cultures
diploid cell cultures, continuous cell cultures
diploid cell cultures last
about 100 generations
continuous cell cultures which are derived from ____ cells last __________
cancer cells, last longer
viroids were discovered in _______ (year)
1971
viroids are
small circular pieces of RNA with no capsid that infects and cause disease in plants
similar pathogenic molecules are known as
fungi
prions
are infectious protein particles that lack nucleic acids and replicate by converting similar normal proteins into new prions
disease caused by prions are
bovine spongiform encephalopathies
disease by prions involve fatal
neurological degeneration, Kuru, Creutzfeldt Jakob Disease, scrapie
term
UV light or x rays can induce conversion of prophage back to lytic phage
lysogeny
viruses are (size)
miniscule nanometers in size
_______ cause most diseases that plagues the world
viruses
extracellular has a _________ which is a protein coat surrounding nucleic acid
varion
intracellular: virus exists as a ________
non enveloped or enveloped?
nucleic acid
non enveloped
what is the primary way scientists categorize and classify viruses?
genetic material
capsid is composed of proteinaceous subunits called
capsomeres
Replication of animal viruses-
DNA viruses often _________
RNA viruses often ___________
DNA- often enter the nucleus
RNA- often replicate in the cytoplasm
Environmental factors that contribute to activation of oncogenes (4)
1. uv light
2. radiation
3. carcinogens
4. viruses
viruses cause _____ (%) of human cancers
20-25%
viroids are extremely _______ (size)
(shape) __________
of (DNA or RNA) ____________
small, circular, RNA