The negative staining technique uses what kind of dye solution?
One in which the chromogen is acidic and carries a negative charge (acidic chromogen gives up a hydrogen ion, which leaves it with a negative charge)
How does negative stain work?
The negative charge on the bacterial surface repels the negatively charged chromogen, so the cell remains unstained against a colored background.
What is the negative staining technique used to determine?
Morphology and cellular arrangement in bacteria that are too delicate to withstand heat fixing. Also, where determining the accurate size is crucial, a negative stain can be used because it produces minimal sell shrinkage.
2 types of negative stains