Ch. 3 Microscopy

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College: Second year
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microbiology
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1

lab measurements

micrometers

2

uses visible light as an illumintation source

compound light microscope

3

objective lens power x ocular lens power

total magnification

4

ability to distinguish fine detail

resolution

5

measure of light bending ability of medium

refraction

6

uses a condenser with an opaque disc allowing only light reflected off the specimen

darkfield microscope

7

does not require fixing or staining. focuses on wave peak of light

phase contrast microscope

8

reconstructs 3D images. specimens are stained with flourochrome

confocal microscope

9

objects are magnified 10,000 to 100,000 times. a beam of electrons is passed through sections of the specimen

transmission electron microscopy

10

electron beam is directed at the top of a specimen

scanning electrom microscope

11

a thin metal probe examines the surface of a specimen

scanning tunneling mmicroscopy

12

a metal and diamond probe that moves along the surface of a specimen yielding a 3 dimensional image

atomic force microscopy

13

a thin film spread on the surface of a slide

smear

14

Has a colored ion that is positive, helping it to adhere to bacteria

basic dyes

15

has a negative color ion that is more attracted to the background

acid dyes

16

a field of colorless bacteria are presented against a stained background

megative staining

17

stain used to visualize shape and arrangement of cells

simple stain

18

chemical used to alter intensity or increase size of a stain for visibility

mordant

19

most useful differential stain

Gram stain

20

dye applied to a heat fixed smear in Gram staining

crystal violet

21

primary stain in a Gram stain

crystal violet

22

3rd step of a Gram stain

mordant added

23

2nd step of Gram stain

water rinse

24

3th step in a Gram stain

wash with ethanol or acetone-ethanol solution

25

stains retaining purple, crystal violet dye

Gram-positive

26

red dye applied to colorless microorganisms in a Gram stain

Safranin

27

counterstain in Gram staining

Safranin

28

stains where microorganism appears pink after counterstain

gram negative

29

dye used in acid fast stains

carbol fuschin

30

counterstain in an acid fast stain

methylene blue

31

stain where cells do not decolorize

acid fast stains