microscope

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created 6 years ago by alifrae
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Grade levels:
College: Second year
Subjects:
microbiology
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1

Bright field microscope

Cells dead, not much detail, stain

2

Phase contrast microscope

Living cells,great detail, no stain

3

Dark field microscope

Acid stain, hard to see, see movement, cells alive

4

Which microscope is used to see syphilis

Dark field microscope

5

What cause syphilis

Treponema pallidum

6

Fluorescence microscopy

Uv radiation, tag without dye, light when bind, immunology, antibodies

7

There are two types of electron microscope

Scanning, transmission

8

Transmission microscope

Great detail, kills specimen

9

What are the three different types of stains

Simple, differential, negative

10

Simple stain, use how many dyes?

One

11

Why are simple stains used

Allow to see specimen

12

What are the two differential stain

Gram , acidfast

13

Differential stain use two dyes called

Primary and counter stain

14

what does the gram stain tell?

Thick cell wall or not

15

Gram stain differentiate between

Gram + or gram -

16

Acid fast tell if there is

An extra lipid layer or not

17

Acid fast differentiate between

Acid fast or non acid fast

18

negative stain

Dye doesn't stick, form a sillhouette

19

Basic dye crystal violet

Purple

20

Basic dye safrinon

Pink/red

21

Carbofuschion

Cherry red

22

melachite green

Green, weak dye

23

Methaline blue

Blue

24

Negative/acidic dyes

India ink, black

25

Cell wall function

Protect, cell shape

26

Cell wall shape cocci, bacilli, spiral

Round, rod, wavy

27

What causes walking pneumoniae

Mycoplasma pneumonia

28

Does walking pneumoniae have a cell wall

No

29

What causes anthrax

Bacillus anthracis

30

What does staphlococus aureus cause

Skin/wound infection

31

Primary stain

Stays on cell throughout procedure (if you can see the stain at the end of the the cell wall is thick

32

Moridant

Help primary stain stick,called chemical or a procedure

33

Decolorizer

Wash out the stain if possible

34

Counter stain

Confirms a negative result

35

Describe gram positive layer

Doesn't have a outer membrane layer, peptidoglycan layer thick, inner membrane yes

36

What does a acid fast stain detect

Detect mycobacterium tuberculosis

37

What does moridant heat do

Heat change