Recombinant DNA technology chapter 8

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created 7 years ago by mayers8
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microbiology
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1

Recombinant DNA technology

modern techniques in molecular biology used to manipulate an organism's genes by introducing , eliminating or changing genes

2

Reverse transcriptase

an enzyme that transcribes DNA nucleotides from an RNA template

3

Restriction enzymes

used by bacterial cells to protect against phages by cutting phage DNA into nonfunctional peices

4

Bacterial enzymes

cut DNA molecules only at specific locations / cuts with sticky ends /cuts with blunt ends

5

Vectors

nucleic acid molecules that deliver gene into a cell

6

Polymerase Chain Reaction

producing identical molecules of DNA in vitro

7

PCR STEPS

denaturation, priming, extension

8

denaturation

separation of two strands of DNA

9

priming

addition of nucleotide mixture followed by cooling

10

extension

heating to increase the rate of replication