Organic Molecule Lab Tests

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1

What does Benedict's test for?

Tests for simple sugars

2

When your are using Benedict's test, what is the process to see if sugar is present?

place 10-20 drops of the Benedict's into your test tube. then place in boiling water for at least 5 minutes.

3

If sugar is present when using the Benedict's test, what color will the solution in the test tube be?

The solution changes from blue to green to reddish brown as more cuprous ions are formed.

4

What does Iodine test for?

Tests for starches.

5

When your are using Iodine test, what is the process to see if starch is present?

place 2 drops of iodine in a test tube, then mix

6

If starch is present when using the Iodine test, what color will the solution in the test tube be?

Starches interacts with the iodine to produce a bluish-black color
(a yellowish-brown color indicates that no starch is present)

7

What does Sudan IV test for?

Tests for Lipids

8

When your are using Sudan IV test, what is the process to see if lipids is present?

1. place filter paper on a paper towel.
2. add what you are testing.
3. allow filter paper to dry.
4. cover bottom of watch glass with Sudan IV.
5. using tweezers place filter in the watch glass
for 3 minutes.
6. then rinse for 1 minute.

9

If lipids are present when using the Sudan IV test, what color will the solution in the test tube be?

A red color at the interface of a lipid and Sudan IV dye.

10

What does Biuret test for?

Tests for Proteins

11

When your are using Biuret test, what is the process to see if proteins is present?

add 5 drops of Biuret to each test tube. then mix

12

If proteins are present when using the Biuret test, what color will the solution in the test tube be?

When copper sulfate (CuS04) reacts with peptide bonds in the presence of sodium hydroxide (NaOH), a violet color results.