Exercise 25 Flashcards


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created 10 years ago by creidt
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Chemical Methods of Control: Antimicrobial Drugs
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1

Pasteur

Observed that infecting an animal with Pseudomonas aeruginosa protected the animal against Bacillus anthracis

2

Antibiotic

A substance produced by a microorganism that inhibits other microorganisms

3

Alexander Fleming

Observed antibiosis around a penicillium mold growth on a culture of staphylococci

4

Selman Waksman

Isolated the antibiotic sterptomycin, produced by actinomycete

5

Stretomyces Bacteria

Produce nearly 70% of all antibiotics

6

Antimicrobial drugs

-chemical used internally
-weather natural or synthetic

7

Pathogen

Disease-causing organism

8

Disk-diffusion method

-Disk impregnated with various antimicrobial agents
-Agents diffuse during incubation
-Diffuses from area of high concentration to low

9

Zone of inhibition

-Are around disk that inhibits bacterial growth
-Zone size affected by diffusion rate and growth rate

10

Kirby-Bauer test

-standard test
-test for agar diffusion methods
-uses Mueller-Hinton agar

11

Minimum Inhibitory Concentration (MIC)

determined by testing for bacterial growth in dilutions of the antibiotic in nutrient broth

12

Zone of inhibition is measured in

Millimeters

13

Each filter disk placed on agar is impregnated with

Antibiotic

14

Agar used in the Kirby-Bauer test to test the effectiveness of antibiotics

Muller-Hinton agar plate

15

The paper disks have a symbol which represents________ and a number which represents___________

-Name of antibiotic
-The amount of antibiotic

16

The ________ of the zone of inhibition is measured to determine if an organism is susceptible or resistant to a particular antibiotic

Diameter

17

Plates used for disk diffusion method are inoculated with

a sterile cotton swab

18

Why is it important to tap the filter disk gently before incubating

To prevent it from falling off agar when u flip the plate for incubation and allowing it to stick