Exercise 24 Flashcards


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created 10 years ago by creidt
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Chemical Methods of Control: Disinfectants and Antiseptics
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1

Antimicrobial agents

A variety of chemicals used to control the growth of microbes

2

Disinfectants

Chemical agents used on inanimate objects to lower the level of microbes on their surface

Examples: Lysol kitchen cleaner, bleach

3

Antiseptics

Chemicals used on living tissue to decrease the number of microbes

Examples: Mouthwash, Contact lens solution, Hydrogen peroxide

4

Bactericidal agents

Agents that result in bacterial death

5

Bacteriostatic agent

Agents that cause temporary inhibition of growth

6

Variables to consider in selecting an antimicrobial agent

-pH
-solubility
-toxicity
-organic material present
-cost

7

Decimal Reduction Time (DRT)

Time it takes to kill 90% of the test microbial population

8

American Official Analytical Chemist's use-dilution test

-Standard method for measuring the effectiveness of chemical agents

9

Use-dilution test

-Limited to BACTERICIDAL compounds
-Cannot be used for BACTERIOSTATIC compounds

10

Which bacteria are used for standard use-dilution test?
Three answers

-Staphylococcus aureus
-Salmonella enterica
-Pseudomonas aeruginosa

11

Bacteriolytic

destruction of bacteria

12

Steps involved in use-dilution test

1) Dip metal rings in bacterial culture
2) Allow metal rings to air dry
3) Dip metal rings in chemical agent for 10 min
4) Place metal rings in nutrient broth